Arch Virol (2000) 145: 1067–1079
The genome structure of kyuri green mottle mosaic tobamovirus
and its comparison with that of cucumber green mottle mosaic
, M. Nishiguchi
, M. Murata
, and F. Motoyoshi
Research Institute for Bioresources, Okayama University, Kurashiki, Japan
National Institute of Agrobiological Resources, Tsukuba, Ibaraki, Japan
Accepted January 25, 2000
Summary. The genome of the Y strain of kyuri green mottle mosaic virus
(KGMMV-Y)hasbeen completelysequenced. Itsgenomic structureand sequence
show it to be a typical tobamovirus, that is closest to, but distinct from, that of
cucumber green mottle mosaic tobamovirus (CGMMV). The genomic sequence
of KGMMV-Y was compared in detail with that of the SH strain of CGMMV.
The sequences of their 5
- and 3
-untranslated regions were 74% and 63% iden-
tical. The amino acid sequences of the shorter and longer (read through) RNA
replicase components, movement protein (MP) and coat protein (CP) were 58,
58. 60 and 46% identical, respectively. The KGMMV-Y genome sequence was
also compared partly to that of another strain of KGMMV, KGMMV-C. The CP
sequences of KGMMV-Y and KGMMV-C differed by 20 amino acid residues,
suggesting that their relationship is more distant than the relationship between
CGMMV-SH and CGMMV-W whose CP sequences are identical. The MPs of
KGMMV-Y and KGMMV-C, however, differ only by one amino acid residue,
although three amino acid substitutions are present in the MPs between CGMMV-
SH and CGMMV-W. Two long stretches, one in the RNA replicase and the other
in the MP, were highly conserved in KGMMV and CGMMV.
The sequences reported in this paper have been deposited in the GenBank nucleotide
sequence database under accession numbers AB015144, AB015145 and AB015146.
Pressent address: Faculty of Food Science and Biotechnology, Universiti Putra
Malaysia, Serdang, Malaysia.