The genome organization of the broad bean necrosis virus (BBNV)

The genome organization of the broad bean necrosis virus (BBNV) The genome of the broad bean necrosis virus Oita-isolate (BBNV-O) (RNA1 (6.0 kb), RNA2 (2.8 kb) and RNA3 (2.4 kb)) was cloned and sequenced. Computer analysis indicates that methyltransferase, helicase and RNA-dependent RNA polymerase (RdRp) motifs are present in RNA1. The viral capsid protein (CP) cistron is located at the 5′ terminal end of RNA2 and the Mr of CP (20 K) is close to that determined by SDS-PAGE analysis. An ochre codon (UAA) in the CP cistron is thought to be partially suppressed to produce a large readthrough protein. RNA3 possesses typical motifs of triple gene block proteins, which are also reported in several other plant viruses. The furovirus genome organization and phylogenetic analysis using RdRp and CP amino acid sequences suggest that BBNV is closely related to potato mop-top virus (PMTV), but is relatively distantly related to other furoviruses. The data also suggest that the genus Furovirus should be separated into several genera: the prototypical genus Furovirus , which excludes the following viruses: the PMTV group including BBNV; the beet necro- tic yellow vein virus (BNYVV) group; and the peanut clump virus (PCV) group. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

The genome organization of the broad bean necrosis virus (BBNV)

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Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1998 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050379
Publisher site
See Article on Publisher Site

Abstract

The genome of the broad bean necrosis virus Oita-isolate (BBNV-O) (RNA1 (6.0 kb), RNA2 (2.8 kb) and RNA3 (2.4 kb)) was cloned and sequenced. Computer analysis indicates that methyltransferase, helicase and RNA-dependent RNA polymerase (RdRp) motifs are present in RNA1. The viral capsid protein (CP) cistron is located at the 5′ terminal end of RNA2 and the Mr of CP (20 K) is close to that determined by SDS-PAGE analysis. An ochre codon (UAA) in the CP cistron is thought to be partially suppressed to produce a large readthrough protein. RNA3 possesses typical motifs of triple gene block proteins, which are also reported in several other plant viruses. The furovirus genome organization and phylogenetic analysis using RdRp and CP amino acid sequences suggest that BBNV is closely related to potato mop-top virus (PMTV), but is relatively distantly related to other furoviruses. The data also suggest that the genus Furovirus should be separated into several genera: the prototypical genus Furovirus , which excludes the following viruses: the PMTV group including BBNV; the beet necro- tic yellow vein virus (BNYVV) group; and the peanut clump virus (PCV) group.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 1998

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