The gene encoding anti-müllerian hormone type 2 receptor maps to mouse Chromosome 15

The gene encoding anti-müllerian hormone type 2 receptor maps to mouse Chromosome 15 Brief Data Reports Ma a afi n Iocorporatiog Moose Genome © Springer-Verlag New York Inc. 1998 Mammalian Genome 9, 259-268 (1998). The gene encoding anti-Mi llerian hormone type 2 receptor maps to mouse D15Mit34 q • q q U q U Chromosome 15 D15Mit44 q q 0 q N q 0 Amhr2 • q q O N q q • D15Mit35 • q q 0 • q • q Tetsuo Kunieda, l 2 Izumi Ojika, l Hideki Katoh 30 20 3 2 1 1 1 1 'Faculty of Agriculture, Okayama University, Tsushimanaka, Okayama 700, Japan 2Central Institute for Experimental Animals, Chr 15 Miyamae, Kawasaki 216, Japan Fig. 1. Position of the Amhr2 Received: 8 August 1997 / Accepted: 18 November 1997 locus on the mouse Chrs 15. Localization was determined Mouse Species: by analysis of 59 (PMA x D15Mit34 Anti-Miillerian hormone type 2 receptor MSM)F, x PMA backcross Locus name: 8 5 progeny. (A) Segregation Locus symbol: Amhr2 ± 36 pattern of the Amhr2 locus Map position: Amhr2 is located on mouse Chromosome (Chr) 15: and flanking loci. (B) Partial D15Mit44 Centromere-D1 5Mit34-(8 .5 ± 3.6)-D15Mit44-(3.4 ± 2.4)- linkage map of mouse 3.4 ± 2.4 Amhr2-(3.4 ± 2.4)-D15Mit35 chromosome 15 showing the Amhr2 Method of mapping: Amhr2 was localized by haplotype analysis 3.4 ± 2.4 Amhr2 locus in relation to of 59 intersubspecific backcross progeny derived from mating of D15Mit35 linked genes. The (PMA x MSM)F 1 x PMA [1] mice. MSM is an inbred strain recombination frequency in percentage is shown on the derived from the Japanese wild mouse (Mus musculus molossinus). left. A 730-bp fragment of rat Amhr2 gene [2] Molecular reagents: was amplified from rat ovary RNA by RT-PCR method with a set of primers (5'-GTATCCCCAAACAGGAGGACC-3' and 5'- CATAACCACATTGTTCGCTTT-3'). The amplified fragment was subcloned into pBluescript II vector, confirmed its nucleotide sequence, and used as a probe to determine chromosomal location. Southern blot analysis of Kpnl-digested DNA Allele detection: of the mouse Chr 15 correspond to those of human Chr 12q [8]. detected a 10-kb fragment in MSM DNA and an 8-kb fragment in The present findings provide further evidence for chromosomal PMA DNA. conservation between mouse Chr 15 and human Chr 12q. The mouse Amhr2 gene and its Previously determined homologs: human homolog (AMHRII) have been localized on Chr 15F and References Chr 12g12-q13, respectively, by in situ hybridization [3]. 1. Esaki K, Yasuda Y, Nakamura M, Hayashi H, Ono K (1981) Exp Anim Discussion: Anti-Miillerian hormone (AMH), or Miillerian inhib- 30, 151-155 iting substance, is a member of TGF -13 family secreted by the 2. Baarends WM, van Helmond MJL, Post M, van der Schoot JCM, Sertoli cells of the fetal and adult testis [4]. The secretion of the Hoogerbrugge JW, de Winter JP, Uilenbroek JTJ, Karels B, Wilming AMH results in regression of Miillerian ducts, the anlagen of the LG, Meijers JHC, Themmen APN, Grootegoed JA (1994) Development female reproductive organs including uterus, oviducts, and upper 120, 189-197 portion of the vagina. The AMH type 2 receptor is a serine/ 3. Visser JA, McLuskey A, van Beers T, Weghuis DO, van Kessel AG, threonine kinase with a single transmembrane domain belonging to Grootegoed JA, Themmen AP (1995) Biochem Biophys Res Commun the family of type II receptor for TGF -13 and other related growth 215,1029-1036 4. Cate RL, Mattaliano RJ, Hession C, Tizard R, Farber NM, Cheung A, and differentiation factors [2,5]. Mutations in human and targeted Ninfa EG, Frey AZ, Gash DJ, Chow EP, Fisher RA, Bertonis JM, Torres disruption in mouse of the AMH type 2 receptor gene (Amhr2) G, Waliner BP, Ramachandran KL, Ragin RC, Manganaro TF, cause internal male pseudohermaphrodites, possessing uterus and MacLaughlin DT, Donahoe PK (1992) Cell 45, 685-698 oviducts in addition to a complete male reproductive tract [6,7]. In 5. Teixeira J, He WW, Shah PC, Morikawa N, Lee MM, Catlin EA, the present study, we mapped the Amhr2 gene on the distal region Hudson PL, Wing J, MacLaughlin DT, Donahoe PK (1996) Endocri- of the mouse Chr 15. The finding is consistent with the previous nology 137, 160-165 gene on mouse Chr study reporting the localization of the Amhr2 6. Mishina Y, Rey R, Finegold MJ, Matzuk MM, Josso N, Cate RL, 15F [2]. No particular mutant locus affecting the male reproductive Behringer RR (1996) Genes Dev 10, 2577-2587 system has been localized on this region. Several loci on the region 7. Imbeaud S, Faure E, Lamarre I, Mattr i M-G, di Clemente N, Tizard R, Carr6-Eusebe, Belville C, Tragethon L, Tonkin C, Nelson J, McAuliffe M, Bidat J-M, Lababidi A, Josso N, Cate RL, Picard J-Y (1995) Nat Genet 11, 382-388 8. Huppi K, Siwarski D, Eppig JT, Mock BA (1996) Mamm Genome 6 (Suppl), S256—S270 Correspondence to: T. Kunieda Mammalian Genome Springer Journals

The gene encoding anti-müllerian hormone type 2 receptor maps to mouse Chromosome 15

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Copyright © 1998 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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