The first complete nucleotide sequence of a grapevine virus E variant

The first complete nucleotide sequence of a grapevine virus E variant Arch Virol (2010) 155:1357–1360 DOI 10.1007/s00705-010-0685-1 ANNOTATED SEQUENCE RECORD The first complete nucleotide sequence of a grapevine virus E variant • • • Beatrix Coetzee Hans J. Maree Dirk Stephan Michael-John Freeborough Johan T. Burger Received: 4 March 2010 / Accepted: 29 April 2010 / Published online: 16 May 2010 Springer-Verlag 2010 Grapevine virus E (GVE) was first isolated from a Japanese Based on the available sequence information (GVE Node table grape cultivar (Vitis labrusca cv. Aki Queen and 3404, TvP15 and TvAQ7 sequence data), the following set Pione) and classified as a new member of the genus Viti- of diagnostic primers was designed: GVE-1-For 5 -AA 0 0 virus in 2008 [1]. Although one of the plants used as a TGGAGTCAAAAGCGATCC-3 and GVE-Rev 5 -GTAG source of virus had stem pitting disease, no relationship GGTCAATCAACCAACA-3 . To identify a GVE-infected between GVE and any disease symptoms could be estab- plant, total RNA was isolated from individual plants using a lished. Grapevine virus E is a positive-sense single-stran- CTAB method adapted for grapevine [3]. The extracted ded RNA virus with a genome organization resembling that RNA was used as a template in RT-PCR using the diag- of grapevine virus http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

The first complete nucleotide sequence of a grapevine virus E variant

Loading next page...
 
/lp/springer_journal/the-first-complete-nucleotide-sequence-of-a-grapevine-virus-e-variant-N1Pz9o7pmC
Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-010-0685-1
Publisher site
See Article on Publisher Site

Abstract

Arch Virol (2010) 155:1357–1360 DOI 10.1007/s00705-010-0685-1 ANNOTATED SEQUENCE RECORD The first complete nucleotide sequence of a grapevine virus E variant • • • Beatrix Coetzee Hans J. Maree Dirk Stephan Michael-John Freeborough Johan T. Burger Received: 4 March 2010 / Accepted: 29 April 2010 / Published online: 16 May 2010 Springer-Verlag 2010 Grapevine virus E (GVE) was first isolated from a Japanese Based on the available sequence information (GVE Node table grape cultivar (Vitis labrusca cv. Aki Queen and 3404, TvP15 and TvAQ7 sequence data), the following set Pione) and classified as a new member of the genus Viti- of diagnostic primers was designed: GVE-1-For 5 -AA 0 0 virus in 2008 [1]. Although one of the plants used as a TGGAGTCAAAAGCGATCC-3 and GVE-Rev 5 -GTAG source of virus had stem pitting disease, no relationship GGTCAATCAACCAACA-3 . To identify a GVE-infected between GVE and any disease symptoms could be estab- plant, total RNA was isolated from individual plants using a lished. Grapevine virus E is a positive-sense single-stran- CTAB method adapted for grapevine [3]. The extracted ded RNA virus with a genome organization resembling that RNA was used as a template in RT-PCR using the diag- of grapevine virus

Journal

Archives of VirologySpringer Journals

Published: Aug 1, 2010

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off