Arch Virol (2010) 155:1357–1360 DOI 10.1007/s00705-010-0685-1 ANNOTATED SEQUENCE RECORD The ﬁrst complete nucleotide sequence of a grapevine virus E variant • • • Beatrix Coetzee Hans J. Maree Dirk Stephan Michael-John Freeborough Johan T. Burger Received: 4 March 2010 / Accepted: 29 April 2010 / Published online: 16 May 2010 Springer-Verlag 2010 Grapevine virus E (GVE) was ﬁrst isolated from a Japanese Based on the available sequence information (GVE Node table grape cultivar (Vitis labrusca cv. Aki Queen and 3404, TvP15 and TvAQ7 sequence data), the following set Pione) and classiﬁed as a new member of the genus Viti- of diagnostic primers was designed: GVE-1-For 5 -AA 0 0 virus in 2008 . Although one of the plants used as a TGGAGTCAAAAGCGATCC-3 and GVE-Rev 5 -GTAG source of virus had stem pitting disease, no relationship GGTCAATCAACCAACA-3 . To identify a GVE-infected between GVE and any disease symptoms could be estab- plant, total RNA was isolated from individual plants using a lished. Grapevine virus E is a positive-sense single-stran- CTAB method adapted for grapevine . The extracted ded RNA virus with a genome organization resembling that RNA was used as a template in RT-PCR using the diag- of grapevine virus
Archives of Virology – Springer Journals
Published: Aug 1, 2010
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