Darkness rapidly induces a decline in the stability and translation of the pea Ferredoxin-1 (Fed-1) mRNA in transgenic tobacco. Direct half-life measurement showed that mutation of the (CAUU)4 stabilizes Fed-1 mRNA in the dark. (CAUU)1, a feature more common in plant 5′ UTRs than (CAUU)4, confers slight light-responsive mRNA accumulation. At least three but less than 11 CAUU repeats near the 5′ end of the 5′ UTR are required for full light-responsive accumulation. Furthermore, 26 nt of the 5′ UTR, including the (CAUU)4 repeat, is sufficient to confer a significant ∼2.5-fold increase in light-regulated mRNA accumulation when fused to the 5′ end of a heterologous plant mRNA. A mutation of the (CAUU)4 repeat that compromises light-regulated mRNA stability changes in vitro the accessibility of the region to ribonuclease V1 and ribonuclease A suggesting the geometry formed by the repeat may be important for instability. Finally, dark-induced Fed-1 mRNA instability occurs even when most of the mRNA is retained on polyribosomes, and thus is likely an independent event regulated by darkness.
Plant Molecular Biology – Springer Journals
Published: Mar 24, 2005
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