The Fed-1 (CAUU)4 element is a 5′ UTR dark-responsive mRNA instability element that functions independently of dark-induced polyribosome dissociation

The Fed-1 (CAUU)4 element is a 5′ UTR dark-responsive mRNA instability element that functions... Darkness rapidly induces a decline in the stability and translation of the pea Ferredoxin-1 (Fed-1) mRNA in transgenic tobacco. Direct half-life measurement showed that mutation of the (CAUU)4 stabilizes Fed-1 mRNA in the dark. (CAUU)1, a feature more common in plant 5′ UTRs than (CAUU)4, confers slight light-responsive mRNA accumulation. At least three but less than 11 CAUU repeats near the 5′ end of the 5′ UTR are required for full light-responsive accumulation. Furthermore, 26 nt of the 5′ UTR, including the (CAUU)4 repeat, is sufficient to confer a significant ∼2.5-fold increase in light-regulated mRNA accumulation when fused to the 5′ end of a heterologous plant mRNA. A mutation of the (CAUU)4 repeat that compromises light-regulated mRNA stability changes in vitro the accessibility of the region to ribonuclease V1 and ribonuclease A suggesting the geometry formed by the repeat may be important for instability. Finally, dark-induced Fed-1 mRNA instability occurs even when most of the mRNA is retained on polyribosomes, and thus is likely an independent event regulated by darkness. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

The Fed-1 (CAUU)4 element is a 5′ UTR dark-responsive mRNA instability element that functions independently of dark-induced polyribosome dissociation

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2005 by Springer
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-004-5109-8
Publisher site
See Article on Publisher Site

Abstract

Darkness rapidly induces a decline in the stability and translation of the pea Ferredoxin-1 (Fed-1) mRNA in transgenic tobacco. Direct half-life measurement showed that mutation of the (CAUU)4 stabilizes Fed-1 mRNA in the dark. (CAUU)1, a feature more common in plant 5′ UTRs than (CAUU)4, confers slight light-responsive mRNA accumulation. At least three but less than 11 CAUU repeats near the 5′ end of the 5′ UTR are required for full light-responsive accumulation. Furthermore, 26 nt of the 5′ UTR, including the (CAUU)4 repeat, is sufficient to confer a significant ∼2.5-fold increase in light-regulated mRNA accumulation when fused to the 5′ end of a heterologous plant mRNA. A mutation of the (CAUU)4 repeat that compromises light-regulated mRNA stability changes in vitro the accessibility of the region to ribonuclease V1 and ribonuclease A suggesting the geometry formed by the repeat may be important for instability. Finally, dark-induced Fed-1 mRNA instability occurs even when most of the mRNA is retained on polyribosomes, and thus is likely an independent event regulated by darkness.

Journal

Plant Molecular BiologySpringer Journals

Published: Mar 24, 2005

References

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