The effects of water temperature and hormone treatments on circulating LH and ovulation in tench (Tinca tinca)

The effects of water temperature and hormone treatments on circulating LH and ovulation in tench... The effectiveness of three hormone treatments commonly used for artificial reproduction of tench was evaluated under three thermal regimes, with focus on the need for dopamine antagonist. Mature females were divided into nine groups (n = 8) and gradually exposed, over a 24 h period, to three temperature regimes: cold (18.1 ± 0.02 °C), optimal (22.05 ± 0.03 °C), and warm (26.3 ± 0.01 °C). Each temperature regime comprised three experimental groups injected with one of three hormone treatments: carp pituitary extract (CPE; 3 mg kg−1); [D-Arg6, Pro9, NEt]-sGnRH (10 μg kg−1); and [D-Arg6, Pro9, NEt]-sGnRH (10 μg kg−1) + metoclopramide (20 mg kg−1) (combined treatment). No differences were found between ovulation induction (ovulation rate ≥ 75 %) with sGnRHa alone and with the combined treatment; whereas CPE at cold and warm water temperatures was significantly less effective (P < 0.05) than above mentioned treatments. Administration of sGnRHa alone induced a gradual increase in luteinizing hormone (LH) levels with LH peaks close to ovulation, in contrast to the immediate LH surge with high LH levels throughout the entire study observed with the combined treatment under all thermal regimes. LH levels induced by GnRHa alone were significantly lower (P < 0.05) at all temperatures compared to the combined treatment, with the exception of the final sample at 26 °C, when no difference was recorded. Based on these results we recommend the application of sGnRHa without the addition of dopamine antagonist as a reliable method for inducing ovulation in tench under suboptimal temperature conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Reviews in Fish Biology and Fisheries Springer Journals

The effects of water temperature and hormone treatments on circulating LH and ovulation in tench (Tinca tinca)

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Publisher
Springer Journals
Copyright
Copyright © 2012 by Springer Science+Business Media B.V.
Subject
Life Sciences; Zoology; Freshwater & Marine Ecology
ISSN
0960-3166
eISSN
1573-5184
D.O.I.
10.1007/s11160-012-9265-7
Publisher site
See Article on Publisher Site

Abstract

The effectiveness of three hormone treatments commonly used for artificial reproduction of tench was evaluated under three thermal regimes, with focus on the need for dopamine antagonist. Mature females were divided into nine groups (n = 8) and gradually exposed, over a 24 h period, to three temperature regimes: cold (18.1 ± 0.02 °C), optimal (22.05 ± 0.03 °C), and warm (26.3 ± 0.01 °C). Each temperature regime comprised three experimental groups injected with one of three hormone treatments: carp pituitary extract (CPE; 3 mg kg−1); [D-Arg6, Pro9, NEt]-sGnRH (10 μg kg−1); and [D-Arg6, Pro9, NEt]-sGnRH (10 μg kg−1) + metoclopramide (20 mg kg−1) (combined treatment). No differences were found between ovulation induction (ovulation rate ≥ 75 %) with sGnRHa alone and with the combined treatment; whereas CPE at cold and warm water temperatures was significantly less effective (P < 0.05) than above mentioned treatments. Administration of sGnRHa alone induced a gradual increase in luteinizing hormone (LH) levels with LH peaks close to ovulation, in contrast to the immediate LH surge with high LH levels throughout the entire study observed with the combined treatment under all thermal regimes. LH levels induced by GnRHa alone were significantly lower (P < 0.05) at all temperatures compared to the combined treatment, with the exception of the final sample at 26 °C, when no difference was recorded. Based on these results we recommend the application of sGnRHa without the addition of dopamine antagonist as a reliable method for inducing ovulation in tench under suboptimal temperature conditions.

Journal

Reviews in Fish Biology and FisheriesSpringer Journals

Published: Apr 20, 2012

References

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