The effects of enhanced UV-B radiation on the RCC1 in wheat somatic cells

The effects of enhanced UV-B radiation on the RCC1 in wheat somatic cells Regulator of Chromosome Condensation 1 (RCC1), a Guanine Nucleotide Exchange Factor (GEF) for the small Ran GTPase (Ran-GTP), is localized on chromatin in the nucleus where it mediates the generation of Ran-GTP. The production of Ran-GTP by RCC1 is critical for nuclear envelope formation, nucleocytoplasmic transport, DNA replication, and chromosome-induced mitotic spindle assembly. Most of the previous studies on RCC1 were carried out in mammalian cells. Only few studies on RCC1 in plant cell have been reported. Here we present an investigation on the identification of RCC1 and its location in wheat (Triticum aestivum L.) somatic cells during mitosis using immunoblotting and immunofluorescence. It was assumed that RCC1 was richer in chromatin than the surrounding cytoplasm throughout mitosis. Compared with white light, enhanced UV-B radiation greatly reduced the content of RCC1 (immunoblotting) and its fluorescence intensity. When RCC1 was absent, laser scanning confocal microscope detected the abnormal chromosomes. The results indicate that the participation of RCC1 in cell cycle provides theoretical ground for revealing the mechanism of abnormal mitosis caused by enhanced UV-B radiation and are of practical significance for agricultural production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The effects of enhanced UV-B radiation on the RCC1 in wheat somatic cells

Loading next page...
 
/lp/springer_journal/the-effects-of-enhanced-uv-b-radiation-on-the-rcc1-in-wheat-somatic-V1bjMFED1Z
Publisher
Pleiades Publishing
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443715050076
Publisher site
See Article on Publisher Site

Abstract

Regulator of Chromosome Condensation 1 (RCC1), a Guanine Nucleotide Exchange Factor (GEF) for the small Ran GTPase (Ran-GTP), is localized on chromatin in the nucleus where it mediates the generation of Ran-GTP. The production of Ran-GTP by RCC1 is critical for nuclear envelope formation, nucleocytoplasmic transport, DNA replication, and chromosome-induced mitotic spindle assembly. Most of the previous studies on RCC1 were carried out in mammalian cells. Only few studies on RCC1 in plant cell have been reported. Here we present an investigation on the identification of RCC1 and its location in wheat (Triticum aestivum L.) somatic cells during mitosis using immunoblotting and immunofluorescence. It was assumed that RCC1 was richer in chromatin than the surrounding cytoplasm throughout mitosis. Compared with white light, enhanced UV-B radiation greatly reduced the content of RCC1 (immunoblotting) and its fluorescence intensity. When RCC1 was absent, laser scanning confocal microscope detected the abnormal chromosomes. The results indicate that the participation of RCC1 in cell cycle provides theoretical ground for revealing the mechanism of abnormal mitosis caused by enhanced UV-B radiation and are of practical significance for agricultural production.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 14, 2015

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from Google Scholar, PubMed
Create lists to organize your research
Export lists, citations
Access to DeepDyve database
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off