ISSN 10630740, Russian Journal of Marine Biology, 2016, Vol. 42, No. 1, pp. 58–64. © Pleiades Publishing, Ltd., 2016.
Original Russian Text © T.B. Kovyrshina, I.I. Rudneva, 2016, published in Biologiya Morya.
Coastal marine ecosystems are the most affected by
human activities, since they are exposed to municipal
wastewater and discharges from fields and marine traf
fic. Human activities impact the natural communities
directly at these areas. At the same time coastal water
areas are the most productive and are characterized by
a high degree of biodiversity; thus, first of all, it is nec
essary to assess their state and to determine the pros
pects for their development and management .
The coastal Black Sea ecosystems have great diver
sity and heterogeneity, many water areas are located in
zones of intense economic activity. This enables one to
conduct a comparative analysis of their ecological
state, depending on the anthropogenic load and to
reveal the effects of its longterm impact on the biota.
For these purposes, concurrently with an analysis of
the physical, chemical and toxicological parameters of
water and sediments, ecotoxicological methods,
which enable one to determine the state of the coastal
area of the sea by biota responses, have been used [14,
19, 20, 34]. The ecotoxicological approach is most
valuable, as it provides comprehensive diagnostics of
marine areas, thus defining the risk to human health
from its resources and development of measures to
restore ecosystems [33, 35].
Our studies on the morphophysiological and bio
chemical parameters of the Black Sea fish that inhabit
the Sevastopol waters with various types of complex
contamination revealed diverse responses of organ
isms to the impacts of unfavorable factors . The
biomarkers that characterize the state of oxidative
stress and antioxidant activity in tissues of fish living in
contaminated areas are the most informative [30–32].
It is also of interest to evaluate the effects of chronic
impacts of human activity on the physiological and
biochemical status of fish.
The objective of this work was a comparative study
of the contents of products of protein oxidation and
antioxidant enzymes activity in the blood of the round
gobies that were caught in 2003 and 2012 in the Stre
letskaya, Martynov, and Karantinnaya bays, which are
located in the Sevastopol area (Black Sea) and are
exposed to various kinds of human activities.
The ecological status of the test bays varies consid
erably. The Streletskaya Bay is most susceptible to
human impacts, because of the intense navigation and
recreation that occurs there. Fuel docks and shipyards
are located on its coasts; their drainage, along with the
discharges of two municipial treatment enterprises and
two stormwater sewer systems, considerably pollute
the marine environment [11, 16]. According to several
authors [6, 8], the water and sediments of the Stre
letskaya Bay contain considerably greater amounts of
oil, heavy metals, chlorinated hydrocarbons, nutrients
and suspended matter than the other two bays. At the
same time, according to [6, 8], the sediments of the
Martynov Bay were contaminated to a greater extent
than those of the Karantinnaya Bay, and the surface layers
of the water, in contrast, were not polluted. That was due
to the different locations of the areas and the discharges
of domestic wastewater in Karantinnaya Bay.
The Effects of Coastal Water Pollution of the Black Sea on the Blood
Biomarkers of the Round Goby
1811 (Perciformes: Gobiidae)
T. B. Kovyrshina and I. I. Rudneva
Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences,
pr. Nakhimova 2, Sevastopol, 299011 Russia
email: firstname.lastname@example.org, email@example.com
Received June 4, 2015
activities of antioxidant enzymes and the contents of the oxidized forms of the proteins in the
blood of the Black Sea round goby
which were sampled in the waters of Sevastopol
in 2003 and 2012, were studied. An increase in the values of the tested biomarkers in fish catches of 2012 com
pared to those of 2003 was recorded. The causes for the observed changes are discussed.
antioxidant enzymes, oxidized proteins, serum proteins,
blood, Black Sea