The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in diabetic rats

The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in... Diabetic retinopathy is a metabolic disease and oxidative stress-associated diabetic disorder. The antioxidant plants are important in the treatment of diabetic mellitus. The main purpose of this study was to determine the effect of Amygdalus scoparia on anti-diabetic and antioxidant activity in the photoreceptor layer of diabetic retina. Thirty rats were housed and divided into three groups of 10 rats each. Group I: control group; group II: Diabetic group (induced by IP injection of 50 mg/kg of stereptozotocin); group III: diabetic receiving 200 mg/kg A. scoparia extract 1 month before and 2 months after diabetic-induced. At the end of experiment, the eyes were removed and fixed in glutaraldehyde. Then, the routine preparation of tissue in transmission electron microscope was done. The semi-thin and ultrathin sections were stained with toluidine blue, uranyl acetate, and lead citrate, respectively. Then, all sections were evaluated by light and transmission electron microscopes. Result showed that the major signs of pathology such as outer segment loss, large vacuoles, and disorganization in inner segment, pyknotic, karyolysis, and karyorrhexis were seen in diabetic group. But, the minor signs of pathology such as small vacuole and pyknotic nuclei were obvious in amygdulus scoparia treatment group. Our findings revealed reduction of the damage in A. scoparia group which might have antioxidant activity and caused blood glucose to be reduced in diabetic group. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in diabetic rats

Loading next page...
 
/lp/springer_journal/the-effects-of-amygdalus-scoparia-extract-on-ultrastructure-changes-of-vSuRYTkCRF
Publisher
Springer London
Copyright
Copyright © 2017 by Springer-Verlag London
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
D.O.I.
10.1007/s00580-017-2479-2
Publisher site
See Article on Publisher Site

Abstract

Diabetic retinopathy is a metabolic disease and oxidative stress-associated diabetic disorder. The antioxidant plants are important in the treatment of diabetic mellitus. The main purpose of this study was to determine the effect of Amygdalus scoparia on anti-diabetic and antioxidant activity in the photoreceptor layer of diabetic retina. Thirty rats were housed and divided into three groups of 10 rats each. Group I: control group; group II: Diabetic group (induced by IP injection of 50 mg/kg of stereptozotocin); group III: diabetic receiving 200 mg/kg A. scoparia extract 1 month before and 2 months after diabetic-induced. At the end of experiment, the eyes were removed and fixed in glutaraldehyde. Then, the routine preparation of tissue in transmission electron microscope was done. The semi-thin and ultrathin sections were stained with toluidine blue, uranyl acetate, and lead citrate, respectively. Then, all sections were evaluated by light and transmission electron microscopes. Result showed that the major signs of pathology such as outer segment loss, large vacuoles, and disorganization in inner segment, pyknotic, karyolysis, and karyorrhexis were seen in diabetic group. But, the minor signs of pathology such as small vacuole and pyknotic nuclei were obvious in amygdulus scoparia treatment group. Our findings revealed reduction of the damage in A. scoparia group which might have antioxidant activity and caused blood glucose to be reduced in diabetic group.

Journal

Comparative Clinical PathologySpringer Journals

Published: May 8, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off