The Effect of the Gene Encoding EcoRII DNA Methyltransferase on the Phenotype of Transgenic Tumor Cell Lines of Nicotiana tabacumand the Methylation Level of CpNpG Sequences in Their Genome

The Effect of the Gene Encoding EcoRII DNA Methyltransferase on the Phenotype of Transgenic Tumor... Several tumor cell lines were obtained by transforming Nicotiana tabacumplants with the recombinant Ti plasmid comprising the gene encoding EcoRII DNA methyltransferase (M·EcoRII) and subjected to analysis. The transformed lines differed in their morphology, growth dependence on hormones, and nopaline-synthesizing capacity. Southern blot-hybridization showed that the M·EcoRII gene was present in the cells of all transformed lines. However, genome analysis using polymerase chain reaction with the oligonucleotide primers recognizing 5"-ends of the M·EcoRII gene did not exhibit the full-length copies of the gene. Lower methylation of CpNpG sequences characteristic of all transformed cells could result from the disturbance of one of several plant DNA methyltransferase genes following its homologous recombination with the M·EcoRII gene. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The Effect of the Gene Encoding EcoRII DNA Methyltransferase on the Phenotype of Transgenic Tumor Cell Lines of Nicotiana tabacumand the Methylation Level of CpNpG Sequences in Their Genome

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Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1016751413442
Publisher site
See Article on Publisher Site

Abstract

Several tumor cell lines were obtained by transforming Nicotiana tabacumplants with the recombinant Ti plasmid comprising the gene encoding EcoRII DNA methyltransferase (M·EcoRII) and subjected to analysis. The transformed lines differed in their morphology, growth dependence on hormones, and nopaline-synthesizing capacity. Southern blot-hybridization showed that the M·EcoRII gene was present in the cells of all transformed lines. However, genome analysis using polymerase chain reaction with the oligonucleotide primers recognizing 5"-ends of the M·EcoRII gene did not exhibit the full-length copies of the gene. Lower methylation of CpNpG sequences characteristic of all transformed cells could result from the disturbance of one of several plant DNA methyltransferase genes following its homologous recombination with the M·EcoRII gene.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 10, 2004

References

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