The influence of dose and time of eggs exposure in tannin solution on tench embryonic development, survival of embryos and percentage of larvae hatched were studied. The eggs obtained under controlled conditions from 11 tench females were fertilized with semen from 7 males and then rinsed for 1 h in Woynarovich solution (40 g urea, 30 g NaCl per 10 dm3 of water). Random samples (50 cm3) of the fertilised eggs were placed into three tannin solutions 0.05, 0.1 or 0.15% for 30, 60 or 90 s, then they were transferred to Weiss jars where incubation at 25°C took place. Tannin solution of 0.05% for 30–90 s or 0.1% for 30 s allowed effective unsticking of eggs and resulted in a very high per cent of hatched larvae. Extending the eggs rinsing time to 60 s in 0.10 and 0.15% solutions resulted in a high mortality of embryos during hatching because of hardened egg capsule.
Reviews in Fish Biology and Fisheries – Springer Journals
Published: Nov 12, 2009
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