The effect of sodium azide and 2,4-dinitrophenol on the development of thermotolerance and induction of Hsp101 in cultured Arabidopsis thaliana cells

The effect of sodium azide and 2,4-dinitrophenol on the development of thermotolerance and... Mild heat shock induces the synthesis of heat shock proteins (HSP) protecting the cell from damages during subsequent severe heat shock. The nature of the signal inducing transcription of Hsp genes is poorly investigated. We studied the effects of mitochondrial inhibitors, sodium azide and dinitrophenol (DNP) on the development of induced thermotolerance and induction of Hsp101 and Hsp60 syntheses in the suspension culture of Arabidopsis thaliana. The presence of sodium azide and DNP during mild heat shock was shown to suppress heat-induced synthesis of Hsp101 and development of induced thermotolerance in the A. thaliana cultured cells. Severe heat shock (50°C) resulted in programmed cell death as was evident from reduced cell viability and cytochrome c release. The results obtained permit a supposition that the functional state of mitochondria determines Hsp gene expression in A. thaliana subjected to heat shock. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The effect of sodium azide and 2,4-dinitrophenol on the development of thermotolerance and induction of Hsp101 in cultured Arabidopsis thaliana cells

Loading next page...
 
/lp/springer_journal/the-effect-of-sodium-azide-and-2-4-dinitrophenol-on-the-development-of-Ob0NhCRg1R
Publisher
Springer Journals
Copyright
Copyright © 2008 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S102144370802009X
Publisher site
See Article on Publisher Site

Abstract

Mild heat shock induces the synthesis of heat shock proteins (HSP) protecting the cell from damages during subsequent severe heat shock. The nature of the signal inducing transcription of Hsp genes is poorly investigated. We studied the effects of mitochondrial inhibitors, sodium azide and dinitrophenol (DNP) on the development of induced thermotolerance and induction of Hsp101 and Hsp60 syntheses in the suspension culture of Arabidopsis thaliana. The presence of sodium azide and DNP during mild heat shock was shown to suppress heat-induced synthesis of Hsp101 and development of induced thermotolerance in the A. thaliana cultured cells. Severe heat shock (50°C) resulted in programmed cell death as was evident from reduced cell viability and cytochrome c release. The results obtained permit a supposition that the functional state of mitochondria determines Hsp gene expression in A. thaliana subjected to heat shock.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jan 20, 2011

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off