The effect of salicylic acid on phenylalanine ammonia-lyase and stilbene synthase gene expression in Vitis amurensis Cell Culture

The effect of salicylic acid on phenylalanine ammonia-lyase and stilbene synthase gene expression... Resveratrol is a phytoalexin with antibacterial, antiviral and cancer-preventing effects. The objective of the study was to identify PAL and STS genes of Vitis amurensis Rupr. encoding the phenylalanine ammonia-lyases (PAL) and stilbene synthases (STS), the key enzymes involved in the resveratrol biosynthesis. A V. amurensis Rupr. cell culture characterized by low resveratrol level was chosen as a model object. Salicylic acid (SA), a known secondary metabolism inducing agent, was used for enhancement of resveratrol production in this culture. PAL and STS gene expression was analyzed using the reverse transcription PCR and real-time PCR techniques. SA was originally found to specifically enhance the expression of VaPAL3, VaSTS2, VaSTS3, VaSTS4, VaSTS5, VaSTS6, and VaSTS8 of multigene families VaPAL and VaSTS. The results obtained were compared with the earlier published data on PAL and STS gene expression in the rolB transformed V. amurensis cell cultures characterized by high levels of resveratrol. The effects of SA treatment and the rolB transformation on VaPAL and VaSTS gene expression were found to be considerably different. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The effect of salicylic acid on phenylalanine ammonia-lyase and stilbene synthase gene expression in Vitis amurensis Cell Culture

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710030143
Publisher site
See Article on Publisher Site

Abstract

Resveratrol is a phytoalexin with antibacterial, antiviral and cancer-preventing effects. The objective of the study was to identify PAL and STS genes of Vitis amurensis Rupr. encoding the phenylalanine ammonia-lyases (PAL) and stilbene synthases (STS), the key enzymes involved in the resveratrol biosynthesis. A V. amurensis Rupr. cell culture characterized by low resveratrol level was chosen as a model object. Salicylic acid (SA), a known secondary metabolism inducing agent, was used for enhancement of resveratrol production in this culture. PAL and STS gene expression was analyzed using the reverse transcription PCR and real-time PCR techniques. SA was originally found to specifically enhance the expression of VaPAL3, VaSTS2, VaSTS3, VaSTS4, VaSTS5, VaSTS6, and VaSTS8 of multigene families VaPAL and VaSTS. The results obtained were compared with the earlier published data on PAL and STS gene expression in the rolB transformed V. amurensis cell cultures characterized by high levels of resveratrol. The effects of SA treatment and the rolB transformation on VaPAL and VaSTS gene expression were found to be considerably different.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: May 9, 2010

References

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