The Effect of Hardening Temperature on the Stability of mRNA for α-Subunit of Translational Elongation Factor 1 in Wheat and Barley Seedlings

The Effect of Hardening Temperature on the Stability of mRNA for α-Subunit of Translational... The effects of hardening temperature (4°C) on the stability of total poly(A)-containing mRNA and mRNA for the α-subunit of translational elongation factor 1 were studied in seedlings of wheat (Triticum aestivumL.) and barley (Hordeum vulgareL.) cultivars differing in their frost tolerance. The in vitro(ommp) system, which adequately characterizes the relative mRNA stability in vivowas used for these measurements. The effects of cooling on mRNA stability were shown to be cultivar-specific. The ratio between dominant and recessive vrnand ppdgenes was assumed to determine the cultivar specificity of the mRNA stability. In frost-tolerant winter wheat and winter barley cultivars, cold-induced changes in the mRNA stability were opposite in their direction. In winter wheat, sodium salicylate affected the stability of mRNAs for the α-subunit of translational elongation factor 1 (eEF-1α), Waxy, and phytochrome A in a cultivar-specific manner. Hardening and sodium salicylate similarly affected the stability of mRNA for eEF-1α, which was shown for four cultivars. Thus, the stability of mRNA in the ommpsystem can be considered a new molecular characteristic of the genotype and plant physiological state, which helps to study the post-transcriptional mechanisms of cereal crop adaptation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The Effect of Hardening Temperature on the Stability of mRNA for α-Subunit of Translational Elongation Factor 1 in Wheat and Barley Seedlings

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Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1012504424109
Publisher site
See Article on Publisher Site

Abstract

The effects of hardening temperature (4°C) on the stability of total poly(A)-containing mRNA and mRNA for the α-subunit of translational elongation factor 1 were studied in seedlings of wheat (Triticum aestivumL.) and barley (Hordeum vulgareL.) cultivars differing in their frost tolerance. The in vitro(ommp) system, which adequately characterizes the relative mRNA stability in vivowas used for these measurements. The effects of cooling on mRNA stability were shown to be cultivar-specific. The ratio between dominant and recessive vrnand ppdgenes was assumed to determine the cultivar specificity of the mRNA stability. In frost-tolerant winter wheat and winter barley cultivars, cold-induced changes in the mRNA stability were opposite in their direction. In winter wheat, sodium salicylate affected the stability of mRNAs for the α-subunit of translational elongation factor 1 (eEF-1α), Waxy, and phytochrome A in a cultivar-specific manner. Hardening and sodium salicylate similarly affected the stability of mRNA for eEF-1α, which was shown for four cultivars. Thus, the stability of mRNA in the ommpsystem can be considered a new molecular characteristic of the genotype and plant physiological state, which helps to study the post-transcriptional mechanisms of cereal crop adaptation.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 10, 2004

References

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