The effects of hardening temperature (4°C) on the stability of total poly(A)-containing mRNA and mRNA for the α-subunit of translational elongation factor 1 were studied in seedlings of wheat (Triticum aestivumL.) and barley (Hordeum vulgareL.) cultivars differing in their frost tolerance. The in vitro(ommp) system, which adequately characterizes the relative mRNA stability in vivowas used for these measurements. The effects of cooling on mRNA stability were shown to be cultivar-specific. The ratio between dominant and recessive vrnand ppdgenes was assumed to determine the cultivar specificity of the mRNA stability. In frost-tolerant winter wheat and winter barley cultivars, cold-induced changes in the mRNA stability were opposite in their direction. In winter wheat, sodium salicylate affected the stability of mRNAs for the α-subunit of translational elongation factor 1 (eEF-1α), Waxy, and phytochrome A in a cultivar-specific manner. Hardening and sodium salicylate similarly affected the stability of mRNA for eEF-1α, which was shown for four cultivars. Thus, the stability of mRNA in the ommpsystem can be considered a new molecular characteristic of the genotype and plant physiological state, which helps to study the post-transcriptional mechanisms of cereal crop adaptation.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 10, 2004
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud