The Effect of Amino Acids as Components of Nutrient Medium on the Accumulation of Protoberberine Alkaloids in the Cell Culture of Thalictrum minus

The Effect of Amino Acids as Components of Nutrient Medium on the Accumulation of Protoberberine... We studied the effects of amino acids on the biosynthesis of protoberberine alkaloids. When 5 mM tyrosine was added to the nutrient medium, the content of alkaloids was reduced by 23% and dry weight was only 77% of the control. On the medium with 1 mM L-tryptophan, the content of alkaloids was somewhat increased (by 20%). Other amino acids (sulfur-containing L-cysteine and L-methionine, and also L-proline and L-arginine) did not affect substantially the content of alkaloids. The addition of 1 and 5 mM L-phenylalanine, which is not a primary precursor to alkaloids, induced the accumulation of alkaloids by the 17th day of the growth cycle by 40 and 140%, respectively, as compared to control treatment. The comparison of various phenylalanine concentrations showed that 7 mM phenylalanine added on the 7– 8th day induced the highest accumulation of alkaloids in the culture medium (above 1 g/l). The content of alkaloids and soluble phenolic compounds increased threefold in both the medium and cells. None of the amino acid tested enhanced biomass accumulation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The Effect of Amino Acids as Components of Nutrient Medium on the Accumulation of Protoberberine Alkaloids in the Cell Culture of Thalictrum minus

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2005 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1007/s11183-005-0058-x
Publisher site
See Article on Publisher Site

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