The effects of four aerobic methylotrophic bacteria on the morphogenesis of soft wheat (Triticum aestivum) were studied in vitro using immature embryos as explants. The inoculation of the embryos with methylotrophic bacteria led to their stable colonization with the bacteria. The colonization of the explants with the strains of Methylobacterium sp. D10 and Methylophilus glucoseoxidans stimulated the formation of morphogenic calli and shoots and also promoted development of the regenerated plants. These regenerated plants manifested bright green leaves and a well-developed root system. The colonization of immature wheat embryos with methylotrophic bacteria can be employed as a tool for raising the efficiency of genetic transformation of various wheat cultivars.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 17, 2004
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