Using universal PCR primers, some 80 fragments of retroelement reverse transcriptase genes were isolated from 16 accessions of the three diploid and three derived allotetraploid species of Brassica in the triangle of U. Sequence analysis showed that the Ty1/copia and LINE-like elements were distinct, while a third clade could be sub-divided into Ty3/gypsy, Athila and virus-like branches, providing evidence that there are multiple sub-lineages within this group normally considered to be gypsy-like elements in plants. The parsimony trees showed no branches correlating with the known genome relationships for the six diploid and allotetraploid Brassica species, probably because members of the element families were present in the common ancestor of the Brassica and, unlike other repetitive sequences, there is no evidence for genome-wide homogenization, although convergent evolution or horizontal transfer cannot be ruled out. Southern hybridization suggested some sub-families were amplified in individual species. The data show that retroelement sequence data do not allow inference of phylogeny, but knowledge of evolution of such abundant sequences assists in exploitation and interpretation of data from other species including models with much smaller genomes and may provide markers.
Plant Molecular Biology – Springer Journals
Published: Dec 30, 2004
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera