The growth of the microscopic red alga Porphyridium purpureum was experimentally studied in different culture media and at various levels of water salinity. The ability of this species to recover after cryopreservation was analyzed. It was revealed that the growth rate and generation time of P. purpureum during the exponential growth phase did not significantly differ between cultures on f medium and those on Goldberg’s medium. The species has been found to withstand salinity variations ranging from 8 to 32 vol %. Cryopreservation of P. purpureum cells using three-step freezing with trehalose as a cryoprotectant showed that the dynamics of cell density after thawing insignificantly differed from the control ones.
Russian Journal of Marine Biology – Springer Journals
Published: Aug 30, 2014
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