ISSN 10630740, Russian Journal of Marine Biology, 2013, Vol. 39, No. 3, pp. 208–213. © Pleiades Publishing, Ltd., 2013.
Original Russian Text © R.V. Men’shova, S.P. Ermakova, B.H. Um, T.N. Zvyagintseva, 2013, published in Biologiya Morya.
Brown algae provide a rich and renewable source of
biologically active polysaccharides: alginic acids, lam
inarans, and fucoidans. These compounds possess a
broad spectrum of biological activities and low toxicity
The brown alga
Setchell, 1905 is distributed along the coasts of Japan
and Korea. It belongs to the family Lessoniaceae of
the order Laminariales and provides a source of
polysaccharides of unique structure. It is known that
glucans) are char
acterized by a high content of 1,6linked glucose resi
dues . Previous studies have shown that the lami
naran from the brown alga
weight of 6 kDa) collected at the Pacific coast of Japan
has a branched structure and the 1,3 : 1,6 linkage ratio
is 1.5 : 1 [16, 19]. We isolated laminaran from
collected in May (at the coast of Korea in the Sea
of Japan) that has a molecular weight of about 5 kDa
and a 1,3 : 1,6 linkage ratio of 2.6 : 1 . As a rule, most
of the investigated algae synthesize laminarans with
10% 1,6linked glucose residue content and molecular
weight 3–6 kDa .
According to the literature data, the fucoidan that
was isolated from
(Pacific coast of Japan) is
a highly sulfated (32.3%) fucan . Earlier, we iso
lated a fucoidan from the
in May (Sea of Japan, Korea) that has a sulfate content
of 13.5% and contains, along with the main monosac
charide residue of fucose, small amounts of galactose,
xylose, and mannose .
It is known that the content of polysaccharides and
their characteristics vary depending on the sampling
location and harvest season [12, 20]. The purpose of
the present work was to isolate biologically active
that were collected in
July 2010 at the coast of Korea in the Sea of Japan; to
study the composition and structural characteristics of
the isolated polysaccharides using the chemical and
physicochemical methods; as well as to compare the
results with the literature data, taking into account the
habitat conditions of the alga.
MATERIALS AND METHODS
Reagents and Materials.
Ethanol, sodium hydrox
ide, inorganic acids, and salts were the commercial
products of the Laverna Co. (Russia); ammonium
hydroxide was from Vekton Co. (Russia); standards
(mannose, rhamnose, glucose, galactose, xylose,
fucose, and dextrans: 6, 20, 40, 80 kDa) were from
Sigma (United States).
The sorbents used for chromatography: Poly
chrome1 was from the “Reakhim” Co. (Russia),
MacroPrep DEAE was from BioRad (United
States), DEAEcellulose was from Sigma (United
States), Superdex 75 HR was from American Pharma
cia Biotech AB (United States).
The brown alga
was collected in
July 2010 at the coast of Korea in the Sea of Japan.
The Composition and Structural Characteristics of Polysaccharides
of the Brown Alga
R. V. Men’shova
, S. P. Ermakova
, B. H. Um
, and T. N. Zvyagintseva
Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, Vladivostok, 690022 Russia
Korean Institute of Science and Technology, Gangneung Institute, Gangneung 210340, Korea
Received November 22, 2012
—The present study examines the polysaccharide composition of the brown alga
The major polysaccharide of
is shown to be sodium alginate (15.8% of the dry defatted alga weight).
The yields of laminaran and fucoidan were 1.4% and 1.3% of the dry defatted alga weight, respectively.
The alga contains laminaran (1,3;1,6
glucan) that has a unique structure, a high content of 1,6linked
glucose residues (the ratio of 1,3 : 1,6 linkage is 1.5 : 1), and an unusually high molecular weight (19–
27 kDa). The fucoidan fractions isolated from
are sulfated and acetylated heteropolysaccharides
that differ in their sulfate content and monosaccharide composition.
: brown algae,
, polysaccharides, laminaran, fucoidan, alginate