Arch Virol (2008) 153: 393–396 DOI 10.1007/s00705-007-1102-2 Printed in The Netherlands Annotated Sequence Record The complete sequence of ligustrum necrotic ringspot virus, a novel carlavirus S. W. Scott, M. T. Zimmerman Department of Entomology, Soils, and Plant Sciences, Clemson University, Clemson, SC, U.S.A. Received 20 August 2007; Accepted 17 October 2007; Published online 7 December 2007 # Springer-Verlag 2007 A sample of the shrub Ligustrum japonicum Thunb. veins in a leaf to become yellow. Sap inoculation of displaying necrotic ringspots and line patterns was C. amaranticolor from both inoculated and non-in- received from Lexington County, South Carolina oculated leaves of other species in the host range via the Clemson University Plant Problem Clinic. study did not produce symptoms. Approach grafts The sample was designated LJ. The subsequent vi- of mature, woody C. amaranticolor to other C. ral isolate was designated LJ1CA1 and is main- amaranticolor transferred the symptom as did sap tained as lyophilized tissue of Chenopodium inoculations between plants of C. amaranticolor. amaranticolor and as frozen partially puriﬁed virus Extraction of leaf tissue from this host using 0.1 M stored at 80 C. Bud tissue of sample LJ1 was potassium phosphate buffer, pH 7.5, containing used for sap
Archives of Virology – Springer Journals
Published: Feb 1, 2008
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