Arch Virol (2009) 154:361–364 DOI 10.1007/s00705-008-0291-7 ANNOTATED SEQUENCE RECORD The complete nucleotide sequence and genome structure of potato latent virus Xianzhou Nie Received: 8 September 2008 / Accepted: 27 November 2008 / Published online: 31 December 2008 Government Canada 2008 Introduction time, the complete genome of PotLV was sequenced, characterized and analyzed. Potato is a natural host to members of more than 30 virus species belonging to about 18 genera . Four viruses, including potato virus S (PVS), potato virus M (PVM), Virus materials and methods potato latent virus (PotLV, or Red LaSoda virus), and potato virus P (PVP), have been recognized as members of The PotLV isolate was obtained from A. Murphy of Potato the genus Carlavirus, family Flexiviridae [1, 5, 7, 10]. Research Centre-Agriculture and Agri-Food Canada. The These viruses possess ﬂexuous ﬁlamentous virions of virus was maintained in plants and tubers of the potato cv. 610–700 nm in length and 12–15 nm in diameter . The ‘Russet Burbank’ as described previously . Total RNA from PotLV-infected potato plants was isolated using the genome is a single-stranded, plus-sense RNA with six open reading frames (ORF). LiCl-procedure . The resulting RNA was quantiﬁed and used for RT-PCR-based ampliﬁcation
Archives of Virology – Springer Journals
Published: Feb 1, 2009
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