Arch Virol (2009) 154:891–894 DOI 10.1007/s00705-009-0365-1 ANNOTATED SEQUENCE RECORD The complete nucleotide sequence and genome organization of red clover vein mosaic virus (genus Carlavirus) Richard C. Larsen Æ Stephen D. Wyatt Æ Keri L. Druffel Received: 2 February 2009 / Accepted: 12 March 2009 / Published online: 3 April 2009 US Government 2009 Red clover vein mosaic virus (RCVMV) incites disease in then harvested 21 days post-inoculation and the virus was pea (Pisum sativum L.) crops, and was recently detected in puriﬁed in a sodium phosphate-sodium citrate buffer using chickpea (Cicer arietinum L.)  and lentil (Lens culin- a protocol established previously . Nucleic acid was aris L.) . Symptoms in pea, lentil and chickpea infected extracted from puriﬁed virions (1 mg/ml) in 300 ll resus- with RCVMV include chlorosis, stunting, rosetting of pension buffer (16.5 mM Na HPO , 1.8 mM sodium 2 4 leaves, and vein clearing Early infection can cause plant citrate, pH 9.0) by the addition of 3 ll 0.1 M dithiothreitol, death in chickpea and lentil. A lack of apical dominance 10 ll proteinase K (10 mg/ml), 30 ll 10% SDS then and a proliferation of axillary buds frequently occurs. Pod incubated for 30
Archives of Virology – Springer Journals
Published: May 1, 2009
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