The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus )

The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus ) Pea streak virus (PeSV) is a member of the genus Carlavirus in the family Betaflexiviridae . Here, the first complete genome sequence of PeSV was determined by deep sequencing of a cDNA library constructed from dsRNA extracted from a PeSV-infected sample and Rapid Amplification of cDNA Ends (RACE) PCR. The PeSV genome consists of 8041 nucleotides excluding the poly(A) tail and contains six open reading frames (ORFs). The putative peptide encoded by the PeSV ORF6 has an estimated molecular mass of 6.6 kDa and shows no similarity to any known proteins. This differs from typical carlaviruses, whose ORF6 encodes a 12- to 18-kDa cysteine-rich nucleic-acid-binding protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus )

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2467-2
Publisher site
See Article on Publisher Site

Abstract

Pea streak virus (PeSV) is a member of the genus Carlavirus in the family Betaflexiviridae . Here, the first complete genome sequence of PeSV was determined by deep sequencing of a cDNA library constructed from dsRNA extracted from a PeSV-infected sample and Rapid Amplification of cDNA Ends (RACE) PCR. The PeSV genome consists of 8041 nucleotides excluding the poly(A) tail and contains six open reading frames (ORFs). The putative peptide encoded by the PeSV ORF6 has an estimated molecular mass of 6.6 kDa and shows no similarity to any known proteins. This differs from typical carlaviruses, whose ORF6 encodes a 12- to 18-kDa cysteine-rich nucleic-acid-binding protein.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 2015

References

  • Family flexiviridae: a case study in virion and genome plasticity
    Martelli, GP; Adams, MJ; Kreuze, JF; Dolja, VV

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