Arch Virol (2008) 153:999–1001 DOI 10.1007/s00705-008-0077-y ANNOTATED SEQUENCE RECORD The complete nucleotide sequence and genome organization of bean yellow disorder virus, a new member of the genus Crinivirus G. Mart´ ın Æ L. Velasco Æ E. Segundo Æ I. M. Cuadrado Æ D. Janssen Received: 19 January 2008 / Accepted: 4 March 2008 / Published online: 4 April 2008 Springer-Verlag 2008 Bean yellow disorder virus (BnYDV) was recently identi- 5 -ends of both plus and minus strands were ampliﬁed with TM ﬁed as the ﬁrst crinivirus that infects members of the family the Smart Race cDNA Ampliﬁcation Kit (Clontech, Leguminosae . The genus Crinivirus belongs to the Mountain View, CA, USA). Cloning and sequencing was family Closteroviridae, a family of arthropod-transmitted done as described . The GenBank accession numbers of plant viruses that primarily infect companion and phloem the complete RNA 1 and RNA 2 sequences reported here for parenchyma cells of their hosts . All members of this BnYDV are EU191904 and EU191905, respectively. virus family have large ss (+) RNA genomes ranging in size The complete RNA 1 and RNA 2 sequences of the from 15 to almost 20 kb, which are encapsidated in long BnYDV genome
Archives of Virology – Springer Journals
Published: May 1, 2008
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