Arch Virol (2010) 155:1717–1719 DOI 10.1007/s00705-010-0769-y ANNOTATED SEQUENCE RECORD The complete genome sequence of the BD variant of grapevine leafroll-associated virus 2 • • Nadia Bertazzon Michele Borgo Elisa Angelini Received: 28 January 2010 / Accepted: 22 July 2010 / Published online: 7 August 2010 Springer-Verlag 2010 Grapevine leafroll-associated virus 2 (GLRaV-2), a mem- The source used for obtaining BD sequences was a plant ber of the genus Closterovirus, is the causal agent of of the table grape cultivar ‘Don Mariano’ from the ampe- leafroll and graft incompatibility in grapevine [4, 7, 10, 12, lographic collection of the ‘‘Centro di Ricerca per la Vi- 13]. Four complete genomic nucleotide sequences of ticoltura’’ in Conegliano (Italy). RNA was extracted from GLRaV-2 isolates have been reported: PN and OR1 from the phloem tissue of woody canes according to MacKenzie the USA [8, 14], RG from California  and 93/955 from et al.  and converted into cDNA as described previously South Africa . The sequences reported for PN and OR1 . The primer pairs for ampliﬁcation of the CP and are almost identical (99.6% nt identity) and have 90% nt HSP70 genes were selected from universal GLRaV-2 identity with 93/955, whereas
Archives of Virology – Springer Journals
Published: Oct 1, 2010
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud