Arch Virol (2011) 156:1489–1492 DOI 10.1007/s00705-011-1033-9 ANNOTATED SEQUENCE RECORD The complete genome sequence of a novel T4-like bacteriophage, IME08 • • • Huanhuan Jiang Xiaofang Jiang Sheng Wang • • • • Cun Li Bin Chen Xiaoping An Zhiqiang Mi Jiankui Chen Yigang Tong Received: 4 March 2011 / Accepted: 11 May 2011 / Published online: 26 May 2011 Springer-Verlag 2011 The genus ‘T4-like viruses’ includes many lytic bacterio- technique on a Solexa machine at BGI (formerly known as phages that infect a wide range of bacteria, including many Beijing Genomics Institute). Sequences were assembled pathogenic ones. These phages are composed of double- using Velvet algorithms  with a k-mer of 31. The stranded DNA genome held in an icosahedral head assembled full-length genome sequence was veriﬁed using attached to a contractile tail with ﬁbers. Sequence analysis other assembly software, including ABYSS  and Soap of the major head gene (g23) has suggested that members . Putative open reading frames within the IME08 full- of genus ‘T4-like viruses’ that infect Escherichia coli can length sequence were predicted using the software Kodon be divided into four major subgroups, represented by T4, (Applied Maths, Sint-Martens-Latem, Belgium), with a RB69, RB49 and
Archives of Virology – Springer Journals
Published: Aug 1, 2011
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