Arch Virol (2005) 150: 815–823
The complete genome sequence of a new necrovirus
isolated from Olea europaea L.
J. M. S. Cardoso
, M. I. E. Clara
and S. Oliveira
Instituto de Ciˆencias Agr´arias Mediterrˆanicas, Universidade de
Departamento de Sanidade Animal e Vegetal, Universidade de
Departamento de Biologia, Universidade de
Received July 9, 2004; accepted October 22, 2004
Published online December 10, 2004
Summary. The complete nucleotide sequence of a virus isolated from Olea
europaea L. (GP isolate), previously identiﬁed as an isolate of Tobacco necrosis
virus D (TNV-D) based on its coat protein sequence, was determined. The viral
RNA genome consists of 3683 nucleotides and contains ﬁve open reading frames.
The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity
with that of an isolate of Olive latent virus 1 (OLV-1) and the coat protein reveals
highest sequence identity with that of TNV-D. Based on the deduced genome or-
ganization and phylogenetic analysis of predicted functional translation products
with that of other necroviruses, the GP isolate genome appears to represent an
example of a new virus arisen by gene exchange and is proposed to be a new
necrovirus, provisionally named Olive mild mosaic virus.
A viral isolate (GP) serologically identiﬁed as tobacco necrosis virus (TNV) was
detected for the ﬁrst time in olive (Olea europaea L.), a crop that has a high
economic importance in Portugal, by F´elix and Clara . According to the
Seventh Report of the International Committee on Taxonomy of Viruses ,
Note: Nucleotide sequence data reported is available in the GenBank database under the
accession number AY616760.