178 Mammalian Genome 9, Brief Data Reports Tokyo 113, and Department of Pathology, The Institute of Medical D8Mit6 Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan 31.6 Received: 27 August 1997 / Accepted: 9 October 1997 (Felis catus) Species: Cat Locus name: Immunoglobulin light chain CX Locus symbol: IGL@ Map position: cat Chr D3 at band p12-pit D8Mit3 12.8 Method of mapping: FISH. A X phage clone, which contains 15-kb inserts hybridizing with the cat IgL CX cDNA fragment (420 bp) , was isolated from a commercially purchased feline geno- DBBordt mic library (Lambda FIX II library, Stratagene). The probe was 11.9 labeled with biotin-16-dUTP by nick translation and mixed with a 10-fold excess of sheared cat genomic DNA to avoid hybridization D8Mgh4 to the repetitive sequences. The signal was detected by FITC- avidin (Boehringer) overlay as described . Prometaphase chro- mosomes were counterstained with propidium iodide and DAPI 27.8 for identification of individual chromosomes. A proposed nomen- clature for cat karyotype  was adapted to localize the gene. Data base deposit information: European Patent Publication Fig. 1. Genetic linkage map of rat Chr 8. Number of the cat IgL CX gene is 0417486A. Numbers on
Mammalian Genome – Springer Journals
Published: Mar 28, 2009
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