The cat immunoglobulin lambda light chain gene maps to Chromosome D3p12–p11

The cat immunoglobulin lambda light chain gene maps to Chromosome D3p12–p11 178 Mammalian Genome 9, Brief Data Reports Tokyo 113, and Department of Pathology, The Institute of Medical D8Mit6 Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan 31.6 Received: 27 August 1997 / Accepted: 9 October 1997 (Felis catus) Species: Cat Locus name: Immunoglobulin light chain CX Locus symbol: IGL@ Map position: cat Chr D3 at band p12-pit D8Mit3 12.8 Method of mapping: FISH. A X phage clone, which contains 15-kb inserts hybridizing with the cat IgL CX cDNA fragment (420 bp) [1], was isolated from a commercially purchased feline geno- DBBordt mic library (Lambda FIX II library, Stratagene). The probe was 11.9 labeled with biotin-16-dUTP by nick translation and mixed with a 10-fold excess of sheared cat genomic DNA to avoid hybridization D8Mgh4 to the repetitive sequences. The signal was detected by FITC- avidin (Boehringer) overlay as described [2]. Prometaphase chro- mosomes were counterstained with propidium iodide and DAPI 27.8 for identification of individual chromosomes. A proposed nomen- clature for cat karyotype [3] was adapted to localize the gene. Data base deposit information: European Patent Publication Fig. 1. Genetic linkage map of rat Chr 8. Number of the cat IgL CX gene is 0417486A. Numbers on http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

The cat immunoglobulin lambda light chain gene maps to Chromosome D3p12–p11

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Publisher
Springer-Verlag
Copyright
Copyright © 1998 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900719
Publisher site
See Article on Publisher Site

Abstract

178 Mammalian Genome 9, Brief Data Reports Tokyo 113, and Department of Pathology, The Institute of Medical D8Mit6 Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan 31.6 Received: 27 August 1997 / Accepted: 9 October 1997 (Felis catus) Species: Cat Locus name: Immunoglobulin light chain CX Locus symbol: IGL@ Map position: cat Chr D3 at band p12-pit D8Mit3 12.8 Method of mapping: FISH. A X phage clone, which contains 15-kb inserts hybridizing with the cat IgL CX cDNA fragment (420 bp) [1], was isolated from a commercially purchased feline geno- DBBordt mic library (Lambda FIX II library, Stratagene). The probe was 11.9 labeled with biotin-16-dUTP by nick translation and mixed with a 10-fold excess of sheared cat genomic DNA to avoid hybridization D8Mgh4 to the repetitive sequences. The signal was detected by FITC- avidin (Boehringer) overlay as described [2]. Prometaphase chro- mosomes were counterstained with propidium iodide and DAPI 27.8 for identification of individual chromosomes. A proposed nomen- clature for cat karyotype [3] was adapted to localize the gene. Data base deposit information: European Patent Publication Fig. 1. Genetic linkage map of rat Chr 8. Number of the cat IgL CX gene is 0417486A. Numbers on

Journal

Mammalian GenomeSpringer Journals

Published: Mar 28, 2009

References

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