The carboxy-terminal half of nonstructural protein 3A is not essential for foot-and-mouth disease virus replication in cultured cell lines

The carboxy-terminal half of nonstructural protein 3A is not essential for foot-and-mouth disease... In foot-and-mouth disease (FMD)-endemic parts of the globe, control is mainly implemented by preventive vaccination with an inactivated purified vaccine. ELISAs detecting antibodies to the viral nonstructural proteins (NSP) distinguish FMD virus (FMDV)-infected animals in the vaccinated population (DIVA). However, residual NSPs present in the vaccines are suspected to be a cause of occasional false positive results, and therefore, an epitope-deleted negative marker vaccine strategy is considered a more logical option. In this study, employing a serotype Asia 1 FMDV infectious cDNA clone, it is demonstrated that while large deletions differing in size and location in the carboxy-terminal half of 3A downstream of the putative hydrophobic membrane-binding domain (deletion of residues 86-110, 101-149, 81-149 and 81-153) are tolerated by the virus without affecting its infectivity in cultured cell lines, deletions in the amino-terminal half (residues 5-54, 21-50, 21-80, 55-80 and 5-149) containing the dimerization and the transmembrane domains are deleterious to its multiplication. Most importantly, the virus could dispense with the entire carboxy-terminal half of 3A (residues 81-153) including the residues involved in the formation of the 3A-3B 1 cleavage junction. The rescue of a replication-competent FMDV variant carrying the largest deletion ever in 3A (residues 81-153) and the fact that the deleted region contains a series of linear B-cell epitopes inspired us to devise an indirect ELISA based on a recombinant 3A carboxy-terminal fragment and to evaluate its potential to serve as a companion diagnostic assay for differential serosurveillance if the 3A-truncated virus is used as a marker vaccine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

The carboxy-terminal half of nonstructural protein 3A is not essential for foot-and-mouth disease virus replication in cultured cell lines

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-016-2805-z
Publisher site
See Article on Publisher Site

References

  • Improvement of a serodiagnostic strategy for foot and mouth disease virus surveillance in cattle under systematic vaccination: a combined system of an indirect ELISA 3ABC with an enzyme linked immunoelectrotransfer blot assay
    Bergmann, IE; Malirat, V; Neitzert, E; Beck, E; Panizzutti, N; Sanchez, C; Falczuk, A
  • Presence of antibodies to non-structural proteins of foot-and-mouth disease virus in repeatedly vaccinated cattle
    Lee, F; Jong, M; Yang, D
  • Recombinant non-structural polyprotein 3AB-based serodiagnostic strategy for FMD surveillance in bovines irrespective of vaccination
    Mohapatra, JK; Pandey, LK; Sanyal, A; Pattnaik, B
  • A mutant of infectious Asia 1 serotype foot-and-mouth disease virus with the deletion of 10-amino-acid in the 3A protein
    Li, S; Gao, M; Zhang, R; Song, G; Song, J; Liu, D; Cao, Y; Li, T; Ma, B; Liu, X; Wang, J
  • Comparative genomics of serotype Asia 1 foot-and-mouth disease virus isolates from India sampled over the last two decades
    Mohapatra, JK; Sanyal, A; Hemadri, D; Tosh, C; Biswas, S; Knowles, NJ; Rasool, TJ; Bandyopadhyay, SK; Pattnaik, B
  • Diagnostic potential of recombinant nonstructural protein 3B to detect antibodies induced by foot-and-mouth disease virus infection in bovines
    Mohapatra, AK; Mohapatra, JK; Pandey, LK; Sanyal, A; Pattnaik, B

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