The C1 inhibitor encoding gene (C1nh) maps to mouse Chromosome 2

The C1 inhibitor encoding gene (C1nh) maps to mouse Chromosome 2 Mammalian Genome 9, Brief Data Reports The Cl inhibitor encoding gene (Clnh) maps to mouse Chromosome 1 Marie Fernandes, 2 Christophe Poirier, Matteo Lener, 3 Hernandez, ) Catherine Bazzali - Mario Tosi, 1 Tommaso Meo''* 'Unite d'Immunogenetique, 25 rue du docteur Roux, Institut Pasteur, 75724 Paris cedex 15, France ZLaboratoire d'Etude du Genome Murin, CNRS/UNSA UMR 6549, Faculte de Medecine, 06107 Nice cedex 2, France 3Unite de Genetique des Mammiferes, 25 rue du docteur Roux, Institut Pasteur, 75724 Paris cedex 15, France Received: 28 July 1997 / Accepted: 10 September 1997 Species: Mouse Locus name: Cl inhibitor gene Locus symbol: C1nh Map position: mapping data were analyzed with RI Manager soft- ware [1]. Distances are expressed in cM with 95% confidence interval. AKXL: Mpmv14-11.11 [3.1-26.1] —Clnh-17.65 [6.8- 34.5]—Rapla-ps2 BXD: D2Ncvs2-5 [0.6-16.91— Clnh-4 [0.5- 13.7] —Mdk of Method mapping: AKXL and BXD Recombinant Inbred Strains sets. Two-color FISH with a human YAC yRP-8B9 (green signal) containing the human homolog of C1nh [2] and a mouse YAC specific for Chr 2 (red signal) [3]. Database deposition information: MGD accession number MGD- Fig. 1. (A) Assignment of the Clnh gene to mouse Chr 2 by fluorescence JNUM-41338. in situ hybridization. (B) Location of Clnh on mouse Chr 2 with BXD Molecular reagents used for mapping: from the cDNA sequence (right) and AKXL (left) RIS sets. (AF010254) we selected a pair of primers allowing amplification References of 440 bp from exon 8 of Clnh: forward 5' GAATTCTT- 1. Manly KF (1993) Mamm Genome 4, 303-313 GACTTCACTTA 3' reverse 5' ATTTGTAGAGTTTGATAGGT 2. Qin S, Nowak NJ, Zhang J, Sait SN, Mayers PG, Higgins MJ, Cheng Y, 3'. Li L, Munroe DJ, Gerhard DS, Weber BH, Brie E, Housman DE, Evans Method of allele detection: polymorphisms between AKR and GA, Shows TB (1996) Proc Natl Acad Sci USA 93, 3149-3154 C57L and between DBA/2 and C57BL/6 were searched by using 3. Mongelard F, Poras I, Usson Y, Batteux B, Robert-Nicoud M, Avner P. FAMA [4]. The forward and reverse primers were end labeled with Vourc'h C (1996) Genomics 38, 432-434 4. Verpy E, Biasotto M, Meo T, Tosi M (1994) Proc Natl Acad Sci USA dyes phosphoramidites 6-FAM and HEX respectively for a second 91, 1873-1877 round of amplification. Heteroduplexes were formed and an A/T 5. Bock SC, Skriver K, Nielsen E, Thogersen HC, Wiman B, Donaldson polymorphism was revealed by mismatch cleavage with the os- VH, Eddy RL, Marrinan J, Radziejewska E, Huber R, et al. (1986) mium tetroxide/piperidine reaction [4]. This polymorphism is a Biochemistry 25, 4292-4301 conservative change within codon Arg 498 at position 1560 of the 6. Kaupmann K, Sendtner M, Jockusch H (1991) Mouse Genome 89, 246 cDNA sequence deposited (A for C57BL/6 and C57L; T for DBA/2 and AKR). Previously identified homologs: human C1NH [5]. Regional localization of Flicl, a Discussion: the human homolog of C1nh has been mapped to calcyclin/S100A6-like gene, to rat 11g13 [5], its location has been refined about 3.5 Mb centromeric to CNTF [21, whose murine homolog has been mapped to Chr 19 Chromosome 7q22-31 by means of FISH [6]. The human 11g12-q 13 genomic region shares conserved syn- teny (from centromere to telomere) with mouse Chrs 2, 19, and 7 F. Prols,' ,* T. Liehr,2,* B. Loser,' B. Rautenstrauss 2 respectively. Our data suggest that the breakpoint of homology between human 11g12-q13 genomic region and mouse Chrs 2 and 'Nephrological Research Department, Medical Clinics IV, Loschgestr. 8, 19 is located between C1NH and CNTF. To date no genes have D-91054 Erlangen, Germany 2Institute of Human Genetics, Schwabachanlage 10, been mapped within this interval, which spans approximately 3.5 D-91054 Erlangen, Germany megabases. Further characterization of this human genomic region should help to understand the evolution of this region. Received: 3 April 1997 / Accepted: 5 September 1997 Species: Rattus norvegicus Acknowledgments: M. Fernandes and C. Poirier are recipients of fellow- Locus name: Flicl ships from the Association pour la Recherche contre le Cancer (ARC) and Locus symbol: Flicl Association Francaise contre les Myopathies (AFM) respectively. M. Lener has been a recipient of an INSERM "poste vert" fellowship. Map position: Chromosome (Chr) 7g22-31 Method mapping: of Fluorescence in situ hybridization (FISH) as * Deceased April 19, 1997 "These authors contributed equally Correspondence to: M. Tosi Correspondence to: B. Rautenstrauss Mammalian Genome Springer Journals

The C1 inhibitor encoding gene (C1nh) maps to mouse Chromosome 2

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Copyright © 1998 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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