The Arabidopsis chloroplast ribosome recycling factor is essential for embryogenesis and chloroplast biogenesis

The Arabidopsis chloroplast ribosome recycling factor is essential for embryogenesis and... To gain insight into the functions of the nuclear-encoded factors involved in chloroplast development, we characterized the high chlorophyll fluorescence and pale green mutant 108-1 (designated as hfp108-1) of Arabidopsis thaliana. Map-based cloning revealed that the mutant contains a tandem repeat of part of the sequence (including 116 nucleotides from 631 to 746 bp downstream of the ATG) of At3g63190, which encodes a chloroplast ribosome recycling factor homologue and was named AtcpRRF. The chloroplasts of hfp108-1 plants contain few internal thylakoid membranes and are severely defective in the accumulation of chloroplast-encoded proteins. In vivo labeling experiments showed a drastic decrease in the synthesis of the chloroplast-encoded proteins, which may be attributed primarily to reduced translation of the corresponding mRNA molecules. The level of the HFP108 transcript was greatly reduced in hfp108-1, so hfp108-1 showed a weak phenotype, and null alleles of HFP108 (hfp108-2) were embryonic lethal. Observations with cleared seeds in the same silique showed that homozygous hfp108-2 seeds were blocked at the heart stage and did not develop further. Thus, these results suggest that AtcpRRF is essential for embryogenesis and chloroplast biogenesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

The Arabidopsis chloroplast ribosome recycling factor is essential for embryogenesis and chloroplast biogenesis

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Publisher
Springer Netherlands
Copyright
Copyright © 2010 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-010-9653-0
Publisher site
See Article on Publisher Site

Abstract

To gain insight into the functions of the nuclear-encoded factors involved in chloroplast development, we characterized the high chlorophyll fluorescence and pale green mutant 108-1 (designated as hfp108-1) of Arabidopsis thaliana. Map-based cloning revealed that the mutant contains a tandem repeat of part of the sequence (including 116 nucleotides from 631 to 746 bp downstream of the ATG) of At3g63190, which encodes a chloroplast ribosome recycling factor homologue and was named AtcpRRF. The chloroplasts of hfp108-1 plants contain few internal thylakoid membranes and are severely defective in the accumulation of chloroplast-encoded proteins. In vivo labeling experiments showed a drastic decrease in the synthesis of the chloroplast-encoded proteins, which may be attributed primarily to reduced translation of the corresponding mRNA molecules. The level of the HFP108 transcript was greatly reduced in hfp108-1, so hfp108-1 showed a weak phenotype, and null alleles of HFP108 (hfp108-2) were embryonic lethal. Observations with cleared seeds in the same silique showed that homozygous hfp108-2 seeds were blocked at the heart stage and did not develop further. Thus, these results suggest that AtcpRRF is essential for embryogenesis and chloroplast biogenesis.

Journal

Plant Molecular BiologySpringer Journals

Published: Jun 4, 2010

References

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