The Arabidopsis AUX1 gene: a model system to study mRNA processing in plants

The Arabidopsis AUX1 gene: a model system to study mRNA processing in plants It is advantageous for an organism to be able to remove aberrant mRNAs that have either been incorrectly transcribed or processed in order to prevent the accumulation of potentially harmful proteins. The selective degradation of nonsense containing transcripts has been described in yeast, Caenorhabiditis elegans and plants. The ease of identification of new mutant alleles in the AUX1 gene of Arabidopsis thaliana has provided a useful system to study novel mutations affecting mRNA stability and pre-mRNA splicing. To date 50 alleles of AUX1 have been identified of which 14 have been characterised at the sequence level. Eight of the characterised alleles encode missense mutations while the others cause nonsense mutations or splicing defects. The 2 splicing mutants identified affect the 5′ or 3′ splice sites and lead to cryptic splicing events resulting in premature stop codons. The AUX1 mRNA levels of the nonsense containing mutants are reduced compared to the wild-type or missense mutants whereas those of a control transcript (SecY) are unaltered. This provides further evidence for a nonsense-mediated mRNA degradation mechanism in plants and provides a system to study the phenomenon further in Arabidopsis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

The Arabidopsis AUX1 gene: a model system to study mRNA processing in plants

Loading next page...
 
/lp/springer_journal/the-arabidopsis-aux1-gene-a-model-system-to-study-mrna-processing-in-wBYjwRb9Kz
Publisher
Springer Journals
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005961303167
Publisher site
See Article on Publisher Site

Abstract

It is advantageous for an organism to be able to remove aberrant mRNAs that have either been incorrectly transcribed or processed in order to prevent the accumulation of potentially harmful proteins. The selective degradation of nonsense containing transcripts has been described in yeast, Caenorhabiditis elegans and plants. The ease of identification of new mutant alleles in the AUX1 gene of Arabidopsis thaliana has provided a useful system to study novel mutations affecting mRNA stability and pre-mRNA splicing. To date 50 alleles of AUX1 have been identified of which 14 have been characterised at the sequence level. Eight of the characterised alleles encode missense mutations while the others cause nonsense mutations or splicing defects. The 2 splicing mutants identified affect the 5′ or 3′ splice sites and lead to cryptic splicing events resulting in premature stop codons. The AUX1 mRNA levels of the nonsense containing mutants are reduced compared to the wild-type or missense mutants whereas those of a control transcript (SecY) are unaltered. This provides further evidence for a nonsense-mediated mRNA degradation mechanism in plants and provides a system to study the phenomenon further in Arabidopsis.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off