The Arabidopsis ACTIN-RELATED PROTEIN 2 (AtARP2) promoter directs expression in xylem precursor cells and pollen

The Arabidopsis ACTIN-RELATED PROTEIN 2 (AtARP2) promoter directs expression in xylem precursor... To investigate the role of the actin cytoskeleton in plant growth and development, we have cloned and determined the DNA sequence of a gene encoding an actin-related protein (arp) from Arabidopsis thaliana (AtARP2) and studied its expression patterns. A. thaliana appears to have only one AtARP2 gene which contains 14 introns, an unusually large number when compared to 4 or 5 introns in the actin genes isolated so far. The predicted protein shows high homology to and shares typical peptide insertions with arp2 proteins from other organisms. The AtARP2 transcript is present in all plant tissues, at a very low level, and is down-regulated by light. Promoter-GUS expression studies showed that the AtARP2 promoter directs activity predominantly in a very small number of cells immediately adjacent to the xylem in all organs. In addition, strong expression was observed in pollen grains. We discuss the potential role of an arp2/3 complex in plant development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

The Arabidopsis ACTIN-RELATED PROTEIN 2 (AtARP2) promoter directs expression in xylem precursor cells and pollen

Loading next page...
 
/lp/springer_journal/the-arabidopsis-actin-related-protein-2-atarp2-promoter-directs-EgYuqgT3Ef
Publisher
Springer Journals
Copyright
Copyright © 1999 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1006247600932
Publisher site
See Article on Publisher Site

Abstract

To investigate the role of the actin cytoskeleton in plant growth and development, we have cloned and determined the DNA sequence of a gene encoding an actin-related protein (arp) from Arabidopsis thaliana (AtARP2) and studied its expression patterns. A. thaliana appears to have only one AtARP2 gene which contains 14 introns, an unusually large number when compared to 4 or 5 introns in the actin genes isolated so far. The predicted protein shows high homology to and shares typical peptide insertions with arp2 proteins from other organisms. The AtARP2 transcript is present in all plant tissues, at a very low level, and is down-regulated by light. Promoter-GUS expression studies showed that the AtARP2 promoter directs activity predominantly in a very small number of cells immediately adjacent to the xylem in all organs. In addition, strong expression was observed in pollen grains. We discuss the potential role of an arp2/3 complex in plant development.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 19, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off