The β-amylase polymorphism of winter common wheat grains

The β-amylase polymorphism of winter common wheat grains The polymorphism of winter common wheat with respect to β-amylase isoenzymes has been analyzed using electrophoresis in polyacrylamide gel (PAAG) buffered with a Tris-glycine system (pH 8.3). Seven β-amylase isoenzymes have been found in wheat varieties and the breeding stocks. Isoenzymes A, B, and C are the most frequent in Russian and Ukrainian varieties (51.7 ± 4.7, 30.7 ± 3.8, and 11.9 ± 2.5%, respectively). Two alleles of the β-Amy-D1 locus of the long arm of chromosome 4D have been identified. The substrate-enzyme affine effect can be used to locate the zones of activity of this enzyme by means of staining for proteins. It has been determined that β-amylase isoenzymes may play a role in the aggregating capacity of the grain protein complex via the formation of S-S bonds. Russian Journal of Genetics Springer Journals

The β-amylase polymorphism of winter common wheat grains

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SP MAIK Nauka/Interperiodica
Copyright © 2012 by Pleiades Publishing, Ltd.
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
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