The activity of exogenous genetic constructs introduced into cells by the technique of ballistic transfection in mouse ontogenesis

The activity of exogenous genetic constructs introduced into cells by the technique of ballistic... We used the method of particle bombardment (ballistic transfection) to introduce β-galactosidase and human dystrophin genes into mouse embryos and skeletal muscles of adult mice. We examined the mechanisms of DNA transfer into skeletal muscle cells, the biological processes accompanying and following this transfer, the susceptibility of various types of muscle cells to transfection, and the duration of expression of and conditions affecting the introduced genes. We have also developed an effective, convenient, and practical methods of skeletal muscle transfection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

The activity of exogenous genetic constructs introduced into cells by the technique of ballistic transfection in mouse ontogenesis

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Publisher
Springer Journals
Copyright
Copyright © 2000 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1007/BF02758914
Publisher site
See Article on Publisher Site

Abstract

We used the method of particle bombardment (ballistic transfection) to introduce β-galactosidase and human dystrophin genes into mouse embryos and skeletal muscles of adult mice. We examined the mechanisms of DNA transfer into skeletal muscle cells, the biological processes accompanying and following this transfer, the susceptibility of various types of muscle cells to transfection, and the duration of expression of and conditions affecting the introduced genes. We have also developed an effective, convenient, and practical methods of skeletal muscle transfection.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Nov 18, 2007

References

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