The 5′ UTR negatively regulates quantitative and spatial expression from the ABI3 promoter

The 5′ UTR negatively regulates quantitative and spatial expression from the ABI3 promoter The involvement of transcription factors Arabidopsis abscisic acid-insensitive3 (ABI3), maize viviparous1 (VP1) and Phaseolus vulgaris ABI3-like factor (PvALF) in the spatial control of storage protein gene expression is well established. However, little insight exists as to how they are themselves regulated. To address this, a 5.15 kb ABI3 upstream sequence including a 4.6 kb full-length promoter and 519 bp of 5′-untranslated region (UTR) was used to drive either β-glucuronidase (GUS) or green fluorescent protein (GFP) expression in Arabidopsis. Expression from the full-length (−4630/+519ABI3) and various 5′-truncated promoters was detected during embryogenesis in all lines, except those transgenic for promoter elements shorter than 364 bp. Two upstream activating regions, −3600 to −2033 and −2033 to −882, enhanced GUS expression in seeds. The −882 to −364 region was sufficient to confer seed-specific expression of GUS when fused to a −64/+6CaMV 35S minimal promoter. Expression from the ABI3 promoter constructs was seed-specific, except in the presence of exogenous abscisic acid (ABA) (>0.3 μM), when GUS expression was detected in seedling roots. Excision of a 405 bp region containing three upstream open reading frames (uORFs) from the 5′-UTR dramatically increased GUS expression and debilitated constraint of reporter expression in roots. Negative regulation of ABI3 expression by the 5′-UTR may involve a post-transcriptional mechanism analogous to that of tumor suppressor genes which also bear long, uORF-containing, 5′-UTRs, or through interactions with RNA-binding proteins. Plant Molecular Biology Springer Journals

The 5′ UTR negatively regulates quantitative and spatial expression from the ABI3 promoter

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Kluwer Academic Publishers
Copyright © 2004 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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