The 5′ untranslated region of potato SBgLR gene contributes to pollen-specific expression

The 5′ untranslated region of potato SBgLR gene contributes to pollen-specific expression Planta (2017) 246:389–403 DOI 10.1007/s00425-017-2695-7 ORIGINAL ARTICLE The 5 untranslated region of potato SBgLR gene contributes to pollen-specific expression 1,2 1,3 1 1 1 • • • • Yujie Chang Min Yan Jingjuan Yu Dengyun Zhu Qian Zhao Received: 22 December 2016 / Accepted: 12 April 2017 / Published online: 25 April 2017 Springer-Verlag Berlin Heidelberg 2017 Abstract important role in pollen-specific expression. Using yeast Main conclusion The 5 UTR of SBgLR enhances gene one-hybrid screening, we isolated a DNA-binding with one expression by regulating both its transcription and finger (Dof) protein gene (StDof23) and an AT-hook motif translation. nuclear-localized (AHL) protein gene (StAHL)frompotato pollen. Further investigation indicated that StDof23 inter- SBgLR (Solanum tuberosum genomic lysine rich) is a pol- acted with and positively regulated the ?31 to ?60 region; len-specific gene in Solanum tuberosum that encodes a moreover, StAHL interacted with and negatively regulated microtubule-associated protein. The region from -85 to the -49 to ?60 region. These results demonstrate that the ?180 (transcription start site at ?1) was determined to be 5 UTR not only enhanced gene expression but also altered critical for specific expression in pollen grains. Transient the tissue-specific expression pattern by regulating both and stable http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Planta Springer Journals

The 5′ untranslated region of potato SBgLR gene contributes to pollen-specific expression

Loading next page...
 
/lp/springer_journal/the-5-untranslated-region-of-potato-sbglr-gene-contributes-to-pollen-SKjMja17KU
Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Springer-Verlag Berlin Heidelberg
Subject
Life Sciences; Plant Sciences; Agriculture; Ecology; Forestry
ISSN
0032-0935
eISSN
1432-2048
D.O.I.
10.1007/s00425-017-2695-7
Publisher site
See Article on Publisher Site

Abstract

Planta (2017) 246:389–403 DOI 10.1007/s00425-017-2695-7 ORIGINAL ARTICLE The 5 untranslated region of potato SBgLR gene contributes to pollen-specific expression 1,2 1,3 1 1 1 • • • • Yujie Chang Min Yan Jingjuan Yu Dengyun Zhu Qian Zhao Received: 22 December 2016 / Accepted: 12 April 2017 / Published online: 25 April 2017 Springer-Verlag Berlin Heidelberg 2017 Abstract important role in pollen-specific expression. Using yeast Main conclusion The 5 UTR of SBgLR enhances gene one-hybrid screening, we isolated a DNA-binding with one expression by regulating both its transcription and finger (Dof) protein gene (StDof23) and an AT-hook motif translation. nuclear-localized (AHL) protein gene (StAHL)frompotato pollen. Further investigation indicated that StDof23 inter- SBgLR (Solanum tuberosum genomic lysine rich) is a pol- acted with and positively regulated the ?31 to ?60 region; len-specific gene in Solanum tuberosum that encodes a moreover, StAHL interacted with and negatively regulated microtubule-associated protein. The region from -85 to the -49 to ?60 region. These results demonstrate that the ?180 (transcription start site at ?1) was determined to be 5 UTR not only enhanced gene expression but also altered critical for specific expression in pollen grains. Transient the tissue-specific expression pattern by regulating both and stable

Journal

PlantaSpringer Journals

Published: Apr 25, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off