Mammalian Genome 9, Brief Data Reports 95 s:* ff:::* HUMACACYA (8] MACPLDQAIGLLVAIFH SGREQDKETLSI=LIELIQIELTIG (45) TCALC (7] (45 ) Tclone15  1PPGSONRLLVAI)EEICYSWMGDKRTLSR10ELKELIQESLTIG (43) Flicl MPPGSGE3tLLVAIFHCYSGIQGD108TLSKXEL1ZLIQIELTIG (43) Fig. 1. Sequence comparison of human , murine , and rat Tclone15 and Flicl amino acid sequences show the high interspecies homologies of the human and murine calcyclin/SI00A6 amino acid sequences HUXACACYA  SALQDAEIARL!l^DLDRN1mQEVNFQEYVTFLGALALIYNEA.LKG (90) to rat Tclone15 and rat Flicl. The asterisks indicate LQALAL D1CALC [71 SKLQDAE IAPLMDDLDRNKDQEVNFQEYVAP IYNEALK (89) differences between the rat Tclonel5/Flicl sequence Tclone15  ARLQDAEIARLI®DLDR2 DQEVNFQEYVAP LGALAL IYNEALK (87) to either the human or murine calcyclin/S100A6 Flicl AICLQDAEIARLNDDLDRN1mQEVNBQEYVAPLGALALIYNEALK (87) amino acid sequence. described in [1,2] was performed on chromosomes derived from human protein . The differences between the sequences are male fibroblast rat cell line RAT 1 . predominantly found within the first nine amino acids of the N- Molecular reagents: The gene expression pattern of proliferating terminal region (Fig. 1). Owing to the 100% sequence identity of and terminally differentiating, tube-forming rat microvascular en- the independently isolated rat clones, Flicl and Tclone 15, a clon- dothelial cells was investigated by means of the differential display ing or sequencing artefact can be excluded, indicating that the method . With
Mammalian Genome – Springer Journals
Published: Mar 28, 2009
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