Tomato plants stably expressing the tetR gene were transformed with the β-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also demonstrated in fruit. Activation of the Triple-Op promoter reduced expression of the downstream hygromycin phosphotransferase II (hptII) gene. Transcriptional interference was therefore observed between tandem genes within T-DNA. Such interference may occur in other T-DNAs, but would not be readily observed without an upstream promoter with controllable activity.
Plant Molecular Biology – Springer Journals
Published: Sep 29, 2004
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