TATA sequence requirements for the initiation of transcription for an RNA polymerase II in vitro transcription system from Nicotiana tabacum

TATA sequence requirements for the initiation of transcription for an RNA polymerase II in vitro... This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two multiple mutations in tobacco, Drosophila, and HeLa in vitro systems. Although the relative transcriptional activities varied among these promoters, all three systems gave similar results. These results suggest the functional conservation of TATA sequence requirements for in vitro transcription systems among plants, insects, and human http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

TATA sequence requirements for the initiation of transcription for an RNA polymerase II in vitro transcription system from Nicotiana tabacum

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1006056128129
Publisher site
See Article on Publisher Site

Abstract

This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two multiple mutations in tobacco, Drosophila, and HeLa in vitro systems. Although the relative transcriptional activities varied among these promoters, all three systems gave similar results. These results suggest the functional conservation of TATA sequence requirements for in vitro transcription systems among plants, insects, and human

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

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