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/lp/springer_journal/targets-of-the-wrky53-transcription-factor-and-its-role-during-leaf-Nuh002Cdn0
- Publisher
- Kluwer Academic Publishers
- Copyright
- Copyright © 2004 by Kluwer Academic Publishers
- Subject
- Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
- ISSN
- 0167-4412
- eISSN
- 1573-5028
- D.O.I.
- 10.1007/s11103-004-2142-6
- Publisher site
- See Article on Publisher Site
Abstract
Arabidopsis WRKY proteins comprise a family of plant specific zinc-finger-type transcription factors involved in the regulation of gene expression during pathogen defense, wounding, trichome development, and senescence. To understand the regulatory role of the senescence-related WRKY53 factor, we identified target genes of this transcription factor by a pull down assay using genomic DNA and recombinant WRKY53 protein. We isolated a number of candidate target genes including other transcription factors, also of the WRKY family, stress- and defence related genes, and senescence-associated genes (SAGs). WRKY53 protein could bind to these different promoters in vitro and in vivo and it could act either as transcriptional activator or transcriptional repressor depending on the sequences surrounding the W-boxes. Overexpression, RNAi and knock-out lines showed accelerated and delayed senescence phenotypes, respectively, and exhibited altered expression levels of the target genes. WRKY53 can be induced by H2O2 and can regulate its own expression in a negative feed back loop. Our results suggest that WRKY53 acts in a complex transcription factor signalling network regulating senescence specific gene expression and that hydrogen peroxide might be involved in signal transduction.
Journal
Plant Molecular Biology – Springer Journals
Published: Dec 30, 2004
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References
-
Gene expression and the thiol redox stateArrigo, A.P.
-
In planta Agrobacteriummediated transformation of adult Arabidopsis thaliana plants by vacuum infiltrationBechtold, N.; Pelletier, G.
-
Leaf senescence: physiology and molecular biologyBiswal, B.; Biswal, U.C.
-
Last exit: senescence, abscission, and meristem arrest in ArabidopsisBleecker, A.B.; Patterson, S.E.
-
The molecular biology of leaf senescenceBuchanan-Wollaston, V.
-
The molecular analysis of leaf senescence-a genomics approachBuchanan-Wollaston, V.; Earl, S.; Harrison, E.; Mathas, E.; Navabpour, S.; Page, T.; Pink, D.
-
Differential expression of a senescence-enhanced metallothionein gene in Arabidopsis in response to isolates of Peronospora parasitica and Pseudomonas syringaeButt, A.; Mousley, C.; Morris, K.; Beynon, J.; Can, C.; Holub, E.; Greenberg, J.T.; Buchanan-Wollaston, V.
-
Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stressesChen, W.; Provart, N.J.; Glazebrook, J.; Katagiri, F.; Chang, H.S.; Eulgem, T.; Mauch, F.; Luan, S.; Zou, G.; Whitham, S.A.; Budworth, P.R.; Tao, Y.; Xie, Z.; Chen, X.; Lam, S.; Kreps, J.A.; Harper, J.F.; Si-Ammour, A.; Mauch-Mani, B.; Heinlein, M.; Kobayashi, K.; Hohn, T; Dangl, J.L.; Wang, X.; Zhu, T.
-
Regulation of the Arabidopsis transcriptome by oxidative stressDesikan, R.; Mackerness, S.A.H.; Hancock, J.T.; Neill, S.J.
-
Expression profile of the WRKY gene superfamily during plant defenceDong, J.; Chen, C.; Chen, Z.
-
The WRKY superfamily of plant transcription factorsEulgem, T.; Rushton, P.J.; Robatzek, S.; Somssich, I.E.
-
Inhibition of leaf senescence by autoregulated production of cytokininGan, S.; Amasino, R.M.
-
Ethylene regulates the timing of leaf senescence in ArabidopsisGrbic, V.; Bleecker, A.B.
-
Programmed senescence of plant organsHadfield, K.A.; Bennett, A.B.
-
Leaf senescence in Brassica napus: expression of genes encoding pathogenesis-related proteinsHanfrey, C.; Fife, M.; Buchanan-Wollaston, V.
-
Active repression mechanisms of eukaryotic transcription repressorsHanna-Rose, W.; Hanson, U.
-
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformationHellens, R.P.; Edwards, E.A.; Leyland, N.R.; Bean, S.; Mullineaux, P.M.
-
Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in ArabidopsisHensel, L.L.; Grbic, V.; Baumgarten, D.A.; Bleecker, A.D.
-
Identification of a transcription factor specifically expressed at the onset of leaf senescenceHinderhofer, K.; Zentgraf, U.
-
Integrated control of seed maturation and germination programs by activator and repressor function of Viviparous1 of MaizeHoecker, U.; Vasil, I.K.; McCarty, D.R.
-
GUS fusions: b-glucuronidase as a sensitive and versatile gene fusion marker in higher plantsJefferson, R.A.; Kavanagh, T.A.; Bevan, M.W.
-
Delayed leaf senescence in ethylenedeficient ACC-oxidase antisense tomato plants: molecular and physiological analysisJohn, I.; Drake, R.; Farrell, A.; Cooper, W.; Lee, P.; Horton, P.; Grierson, D.
-
TRANSPARENT TESTA GLABRA2, a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factorJohnson, C.S.; Kolevski, B.; Smyth, D.R.
-
Involvement of the antioxidative system in tomato response to fusaric acid treatmentKuzniak, E.; Patykowski, J.; Urbanek, H.
-
Functional analysis of regulatory sequences controlling PR-1 gene expression in ArabidopsisLebel, E.; Heifetz, P.; Thorne, L.; Uknes, S.; Ryals, J.; Ward, E.
-
The WRKY70 transcription factor: A node of convergence of jasmonate-mediated and salicylate-mediated signals in plant defenseLi, J.; Brader, G.; Palva, E.T.
-
Molecular analysis of natural leaf senescence in Arabidopsis thalianaLohman, K.N.; Gan, S.; John, M.C.; Amasino, R.M.
-
The transcriptome of Arabidopsis thaliana during systemic acquired resistanceMaleck, K.; Levine, A.; Eulgem, T.; Morgan, A.; Schmid, J.; Lawton, K.A.; Dangl, J.L.; Dietrich, R.A.
-
Senescenceassociated gene expression during ozone-induced leaf senescence in ArabidopsisMiller, J.D.; Arteca, R.N.; Pell, E.J.
-
The molecular genetic analysis of leaf senescenceNam, H.G.
-
Expression of senescence-enhanced genes in response to oxidative stressNavabpour, S.; Morris, A.; Allen, R.; Harrison, E.; Mackerness, S.A.H.; Buchanan-Wollaston, V.
-
Identification of a promoter region responsible for senescence-specific expression of SAG12Noh, Y.S.; Amasino, R.M.
-
Senescence mechanismsNoode´n, L.D.; Guiame´ t, J.J.; John, I.
-
Transcriptional control and role of silencers in transcriptional regulation in eukaryotesOgbourne, S.; Antalis, T.M.
-
A senescence-associated gene of Arabidopsis thaliana is distinctively regulated during natural and artificially induced leaf senescenceOh, S.; Lee, S.Y.; Chung, I.K.; Lee, C.H.; Nam, H.G.
-
Loss of stress-induced expression of catalase3 during leaf senescence in Arabidopsis thaliana is restricted to oxidative stressOrendi, G.; Zimmermann, P.; Baar, C.; Zentgraf, U.
-
Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in ArabidopsisPark, J.H.; Oh, S.A.; Kim, Y.H.; Woo, H.R.; Nam, H.G.
-
Molecular aspects of leaf senescenceQuirino, B.F.; Noh, Y.S.; Himelblau, E.; Amasino, R.M.
-
A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence and defense-related processesRobatzek, S.; Somssich, I.E.
-
Targets of AtWRKY6 regulation during plant senescence and pathogen defenseRobatzek, S.; Somssich, I.E.
-
Interaction of elicitorinduced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genesRushton, P.J.; Torres, J.T.; Parniske, M.; Wernert, P.; Hahlbrock, K.; Somssich, I.E.
-
Signal transduction in maize and Arabidopsis mesophyll protoplastsSheen, J.
-
Senescence and aging in plantsVan Staden, J.; Cook, E.L.; Noode´n, L.D.
-
Isolation of target gene promoter/enhancer sequences by whole genome PCR methodWatson, D.K.; Kitching, R.; Vary, C.; Kola, I.; Seth, A.
-
A comparison of the expression patterns of several senescence-associated genes in response to stress and hormone treatmentWeaver, L.M.; Gan, S.; Quirino, B.F.; Amasino, R.M.
-
A mini binary vector series for plant transformationXiang, C.B.; Han, P.; Lutziger, I.; Wang, K.; Oliver, D.J.
-
The developmental transition to flowering represses ascorbate peroxidase activity and induces enzymatic lipid peroxidation in leaf tissue in Arabidopsis thalianaYe, Z.Z.; Rodriguez, R.; Tran, A.; Hoang, H.; de los Santos, D.; Brown, S.; Vellanoweth, R.L.
-
Senescence related gene expression profiles of rosette leaves of Arabidopsis thaliana: leaf age versus plant ageZentgraf, U.; Kolb, D.; Laun, T.; Rentsch, D.
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