Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography–tandem mass spectrometry

Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase... Lipidomics is the area of study dedicated to the comprehensive analysis and characterization of the functions and metabolism of lipids in biological samples. One of the most comprehensively studied classes of lipids is polyunsaturated fatty acids (PUFAs). Eicosanoids are a series of bioactive lipid mediators that are metabolized from PUFAs and generated majorly from the precursor arachidonic acid. This study identified the profiles of target eicosanoids after acute exposure to arsenic. The principle objective was to determine and validate 10 eicosanoids in mouse serum using on-line solid-phase extraction integrated with liquid chromatography–electrospray tandem mass spectrometry. Intra-day and inter-day repeatability was 82.4–119.2 and 86.7–124.4%, respectively. The limit of detection and limit of quantification were from 0.003 to 0.288 ng/mL and from 0.009 to 0.962 ng/mL, respectively. The levels of 7 of the 10 eicosanoids—namely 8-isoPGF2α, PGF2α, PGE2, 13(s)-HODE, 15(s)-HETE, 12(s)-HETE, and 5(s)-HETE—in mouse serum significantly and dose-dependently increased after arsenic exposure compared with the levels in the vehicle control group. To our knowledge, this is the first study to quantify eicosanoids in mouse serum. This approach provides simple sample preparation, small sample volumes, and a precise and accurate method for determining changes in the profile of these eicosanoids in mouse serum after acute exposure to arsenic. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Toxicology Springer Journals

Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography–tandem mass spectrometry

Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography–tandem mass spectrometry

Arch Toxicol (2017) 91:3079–3091 DOI 10.1007/s00204-017-1937-6 INORGANIC COMPOUNDS Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography–tandem mass spectrometry 1 2 2 1 Chao-Yu Chen  · Pinpin Lin  · Ming-Hsien Tsai  · Hui-Ling Lee   Received: 8 September 2016 / Accepted: 12 January 2017 / Published online: 16 February 2017 © Springer-Verlag Berlin Heidelberg 2017 Abstract Lipidomics is the area of study dedicated to serum. This approach provides simple sample preparation, the comprehensive analysis and characterization of the small sample volumes, and a precise and accurate method functions and metabolism of lipids in biological samples. for determining changes in the profile of these eicosanoids One of the most comprehensively studied classes of lipids in mouse serum after acute exposure to arsenic. is polyunsaturated fatty acids (PUFAs). Eicosanoids are a series of bioactive lipid mediators that are metabolized Keywords Mouse serum · On-line SPE LC-MS/MS · from PUFAs and generated majorly from the precursor ara- Eicosanoids · Arsenic chidonic acid. This study identified the profiles of target eicosanoids after acute exposure to arsenic. The principle objective was to determine and validate 10 eicosanoids in Introduction mouse serum using on-line solid-phase extraction inte- grated with liquid chromatography–electrospray tandem Lipids, as structure builders, play a crucial role in messen- mass spectrometry. Intra-day and inter-day repeatability ger molecules (Rolim et al. 2015). The omics sciences, such was 82.4–119.2 and 86.7–124.4%, respectively. The limit as genomics, proteomics, metabolomics and lipidomics, are of detection and limit of quantification were from 0.003 to areas of systems biology. Recent successes...
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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Springer-Verlag Berlin Heidelberg
Subject
Biomedicine; Pharmacology/Toxicology; Occupational Medicine/Industrial Medicine; Environmental Health; Biomedicine, general
ISSN
0340-5761
eISSN
1432-0738
D.O.I.
10.1007/s00204-017-1937-6
Publisher site
See Article on Publisher Site

Abstract

Lipidomics is the area of study dedicated to the comprehensive analysis and characterization of the functions and metabolism of lipids in biological samples. One of the most comprehensively studied classes of lipids is polyunsaturated fatty acids (PUFAs). Eicosanoids are a series of bioactive lipid mediators that are metabolized from PUFAs and generated majorly from the precursor arachidonic acid. This study identified the profiles of target eicosanoids after acute exposure to arsenic. The principle objective was to determine and validate 10 eicosanoids in mouse serum using on-line solid-phase extraction integrated with liquid chromatography–electrospray tandem mass spectrometry. Intra-day and inter-day repeatability was 82.4–119.2 and 86.7–124.4%, respectively. The limit of detection and limit of quantification were from 0.003 to 0.288 ng/mL and from 0.009 to 0.962 ng/mL, respectively. The levels of 7 of the 10 eicosanoids—namely 8-isoPGF2α, PGF2α, PGE2, 13(s)-HODE, 15(s)-HETE, 12(s)-HETE, and 5(s)-HETE—in mouse serum significantly and dose-dependently increased after arsenic exposure compared with the levels in the vehicle control group. To our knowledge, this is the first study to quantify eicosanoids in mouse serum. This approach provides simple sample preparation, small sample volumes, and a precise and accurate method for determining changes in the profile of these eicosanoids in mouse serum after acute exposure to arsenic.

Journal

Archives of ToxicologySpringer Journals

Published: Feb 16, 2017

References

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