Systemic gene delivery into soybean by simple rub-inoculation with plasmid DNA of a Soybean mosaic virus -based vector

Systemic gene delivery into soybean by simple rub-inoculation with plasmid DNA of a Soybean... Arch Virol (2009) 154:1575 DOI 10.1007/s00705-009-0459-9 ERRATUM Systemic gene delivery into soybean by simple rub-inoculation with plasmid DNA of a Soybean mosaic virus-based vector Jang-Kyun Seo Æ Hyeok-Geun Lee Æ Kook-Hyung Kim Published online: 24 July 2009 Springer-Verlag 2009 Erratum to: Arch Virol (2009) 154:87–99 adopted for stable expression of target proteins in DOI 10.1007/s00705-008-0286-4 soybean [39, 55a, 61]. However, since these viral vectors were constructed to generate recombinant viral In the published article, some statements should be chan- RNAs by in vitro transcription or biolostic delivery of ged as indicated below. It was a misstatement of fact to cDNA, the costs associated with these procedures may claim that Soybean mosaic virus is a novel gene expression be considerable if viral vectors are to be used to vector for soybean in light of the earlier disclosure pub- evaluate large numbers of genes.’’ lished in Mol Plant Microbe Interact 19:304–312 (2006) by 2. On page 97 lines 7–10 (left panel), The sentence Wang et al. Therefore, the following amendments should ‘‘Moreover, to our knowledge, this is the first report be made to the text. demonstrating the feasibility of using SMV as an efficient expression vector for stable expression in 1. On page 88 lines 26–34 (left panel), the statement soybean.’’ must be corrected as follows ‘‘We have also ‘‘Recently, Bean pod mottle virus (BPMV) and engineered this SMV construct as an efficient expres- Cucumber mosaic virus (CMV) have been engineered sion vector for stable expression in soybean. Although as gene delivery vectors and successfully adopted for it was previously demonstrated that SMV can be used stable expression of target proteins in soybean [39, 60]. as a gene delivery vector [55a], in this study we further However, since these viral vectors were constructed to showed that foreign genes can be systemically deliv- generate recombinant viral RNAs by in vitro tran- ered to soybean by simple rub-inoculation with scription, the costs associated with these procedures plasmid DNA of SMV-based vector.’’ may be considerable if viral vectors are to be used to 3. On page 98 line 60 (right panel), add an additional evaluate large numbers of genes.’’ should be revised as reference as 55a. follows ‘‘Recently, Bean pod mottle virus (BPMV), [55a. Wang L, Eggenberger A, Hill J, Bogdanove AJ Cucumber mosaic virus (CMV) and SMV have been (2006) Pseudomonas syringae effector avrB confers engineered as gene delivery vectors and successfully soybean cultivar-specific avirulence on Soybean mosaic virus adapted for transgene expression but effector avrPto does not. Mol Plant Microbe Interact The online version of the original article can be found under doi:10.1007/s00705-008-0286-4. 19:304–312]. J.-K. Seo  H.-G. Lee  K.-H. Kim (&) Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, South Korea e-mail: kookkim@snu.ac.kr J.-K. Seo  K.-H. Kim Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Systemic gene delivery into soybean by simple rub-inoculation with plasmid DNA of a Soybean mosaic virus -based vector

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Publisher
Springer Vienna
Copyright
Copyright © 2009 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-009-0459-9
Publisher site
See Article on Publisher Site

Abstract

Arch Virol (2009) 154:1575 DOI 10.1007/s00705-009-0459-9 ERRATUM Systemic gene delivery into soybean by simple rub-inoculation with plasmid DNA of a Soybean mosaic virus-based vector Jang-Kyun Seo Æ Hyeok-Geun Lee Æ Kook-Hyung Kim Published online: 24 July 2009 Springer-Verlag 2009 Erratum to: Arch Virol (2009) 154:87–99 adopted for stable expression of target proteins in DOI 10.1007/s00705-008-0286-4 soybean [39, 55a, 61]. However, since these viral vectors were constructed to generate recombinant viral In the published article, some statements should be chan- RNAs by in vitro transcription or biolostic delivery of ged as indicated below. It was a misstatement of fact to cDNA, the costs associated with these procedures may claim that Soybean mosaic virus is a novel gene expression be considerable if viral vectors are to be used to vector for soybean in light of the earlier disclosure pub- evaluate large numbers of genes.’’ lished in Mol Plant Microbe Interact 19:304–312 (2006) by 2. On page 97 lines 7–10 (left panel), The sentence Wang et al. Therefore, the following amendments should ‘‘Moreover, to our knowledge, this is the first report be made to the text. demonstrating the feasibility of using SMV as an efficient expression vector for stable expression in 1. On page 88 lines 26–34 (left panel), the statement soybean.’’ must be corrected as follows ‘‘We have also ‘‘Recently, Bean pod mottle virus (BPMV) and engineered this SMV construct as an efficient expres- Cucumber mosaic virus (CMV) have been engineered sion vector for stable expression in soybean. Although as gene delivery vectors and successfully adopted for it was previously demonstrated that SMV can be used stable expression of target proteins in soybean [39, 60]. as a gene delivery vector [55a], in this study we further However, since these viral vectors were constructed to showed that foreign genes can be systemically deliv- generate recombinant viral RNAs by in vitro tran- ered to soybean by simple rub-inoculation with scription, the costs associated with these procedures plasmid DNA of SMV-based vector.’’ may be considerable if viral vectors are to be used to 3. On page 98 line 60 (right panel), add an additional evaluate large numbers of genes.’’ should be revised as reference as 55a. follows ‘‘Recently, Bean pod mottle virus (BPMV), [55a. Wang L, Eggenberger A, Hill J, Bogdanove AJ Cucumber mosaic virus (CMV) and SMV have been (2006) Pseudomonas syringae effector avrB confers engineered as gene delivery vectors and successfully soybean cultivar-specific avirulence on Soybean mosaic virus adapted for transgene expression but effector avrPto does not. Mol Plant Microbe Interact The online version of the original article can be found under doi:10.1007/s00705-008-0286-4. 19:304–312]. J.-K. Seo  H.-G. Lee  K.-H. Kim (&) Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, South Korea e-mail: kookkim@snu.ac.kr J.-K. Seo  K.-H. Kim Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2009

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