ISSN 10703284, Russian Journal of Coordination Chemistry, 2015, Vol. 41, No. 3, pp. 169–174. © Pleiades Publishing, Ltd., 2015.
Cancer continue to pose a very considerable health
problems threat to significant parts of the population.
Chemotherapeutics has become an important method
in the treatment of cancer cells. Recently, there is a
great interest on the binding of transition metal com
plexes with DNA, owing to their possible applications
as new cancer therapeuticagents and their photo
chemical properties that make them potential
probes of DNA structure and conformation [1–3].
Some authors produced evidence to the apoptosis pro
moting nature of Cd including its ability to induce
DNA fragmentation and chromatin condensation in
kidney, blood, and liver cells [4–6].
There has been considerable interest in the design
and synthesis of complexes with carboxylate ligands
because carboxylates can give rise to different coordi
nation modes with metal ions and increase recogni
tion of the metal’s role in biological systems [7, 8]. The
2,3pyrazinedicarboxylate acid (Pzdc) with a number
of potential donor atoms commonly bound to transi
tion metals by two carboxylic groups together with two
pyrazine nitrogens. 1,10Phenanthroline (Phen) can
coordinate with transition metals and disturb func
tions of biological systems. Phen has long been used as
a component of hemoglobin models with strong coor
dination ability and biological activity [9–11]. A novel
coordination compound has been synthesized under
solvent evaporation. In this paper, complex
acterized by IR, elemental analysis, crystal structure
The article is published in the original.
determination, fluorescence, gel electrophoresis and
Materials and measurements
. All chemicals were of
reagent grade quality obtained from commercial
sources and used without further purification unless
otherwise noted. Elemental analyses (C, H, and N)
were performed on a model Finnigan EA 1112 appara
tus. Infrared spectroscopy using KBr pellets were per
formed on a Nicolet FTIR 470 spectrophotometer.
Fluorescence measurements were performed on a
PerkinElmer LS55 fluorescence spectrofluorometer.
Gel electrophoresis were performed on a JS380A gel
Synthesis of complex I.
Experiments were all re
agent concentration 10 mmol/L, Cd(NO
aqueous solution (10 mL) and Pzdc solution (10 mL)
mixing. With potassium hydroxide solution (0.1 mol/L)
pH value of mixed solution will be adjusted to 4.78. Af
ter about 3 h of stirring, 10 mL of ethanol solution
Phen was added. After about another 3 h of stirring,
the solution was filtrated, and the filtrate was evaporat
ed slowly in the air. Colourless crystals were obtained
after 30 days.
IR spectrum (
): 3077 s, 1671 s, 3498 s, 1496 s,
1516 m, 1310 m, 1629 w, 1356 w.
anal. calcd, %: C, 46.68; H, 3.72; N, 7.26.
Found, %: C, 46.70; H, 3.64; N, 7.30.
Syntheses, Structures, Characterization, and Bioactivities
of New Cd(II) Complex of 2,3Pyrazinedicarboxylate
L. Wang, Y. Y. Li, J. Ge, J. Zhang, S. K. Liang, L. F. Li, C. Ma, and E. J. Gao*
Coordination Chemistry Laboratory, Shenyang Institute of Chemical Technology, Shenyang, Liaoning, 110142 P.R. China
Received June 6, 2014
A novel complex [Cd(Phen)
), where Phen = 1,10phenanthroline, Pzdc =
2,3pyrazinedicarboxylic acid, has been synthesized under room temperature conditions and characterized
by IR spectroscopy, elemental analysis, and Xray singlecrystal structure analysis (CIF file CCDC
no. 1023407). Its mode of interaction with fish sperm DNA (FSDNA) have been determined by fluores
cence. Gel electrophoresis assay demonstrates the ability of complex
to cleave the HL60 DNA. Further
more, apoptosis assay shows treatment with complex results in morphological changes of cancer cell lines.
The results induce apoptosis in JEKO cells.