Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation

Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both... Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus , has a single-stranded DNA genome. TYLCV can induce severe disease symptoms on tomato plants, but other hosts plants such as cucurbits and peppers are asymptomatic. A full-length DNA clone of a Korean TYLCV isolate was constructed by rolling-circle amplification from TYLCV-infected tomatoes in Korea. To assess relative susceptibility of sweet pepper varieties to TYLCV, 19 cultivars were inoculated with cloned TYLCV by agro-inoculation. All TYLCV-infected sweet peppers were asymptomatic, even though Southern hybridization and polymerase chain reaction analysis showed TYLCV genomic DNA accumulation in roots, stems, and newly produced shoots. Southern hybridization indicated that TYLCV replicated and moved systemically from agro-inoculated apical shoot tips to roots or newly produced shoots of sweet peppers. Whitefly-mediated inoculation experiments showed that TYLCV can be transmitted to tomatoes from TYLCV-infected sweet peppers. Taken together, these results indicate that sweet pepper can be a reservoir for TYLCV in nature. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2072-9
Publisher site
See Article on Publisher Site

Abstract

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus , has a single-stranded DNA genome. TYLCV can induce severe disease symptoms on tomato plants, but other hosts plants such as cucurbits and peppers are asymptomatic. A full-length DNA clone of a Korean TYLCV isolate was constructed by rolling-circle amplification from TYLCV-infected tomatoes in Korea. To assess relative susceptibility of sweet pepper varieties to TYLCV, 19 cultivars were inoculated with cloned TYLCV by agro-inoculation. All TYLCV-infected sweet peppers were asymptomatic, even though Southern hybridization and polymerase chain reaction analysis showed TYLCV genomic DNA accumulation in roots, stems, and newly produced shoots. Southern hybridization indicated that TYLCV replicated and moved systemically from agro-inoculated apical shoot tips to roots or newly produced shoots of sweet peppers. Whitefly-mediated inoculation experiments showed that TYLCV can be transmitted to tomatoes from TYLCV-infected sweet peppers. Taken together, these results indicate that sweet pepper can be a reservoir for TYLCV in nature.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2014

References

  • A worldwide survey of tomato yellow leaf curl viruses
    Czosnek, H; Laterrot, H
  • Tomato yellow leaf curl virus , an emerging virus complex causing epidemics worldwide
    Moriones, E; Navas-Castillo, J
  • Detection and molecular characterization of viruses associated with tomato yellow leaf curl disease in cucurbit crops in Jordan
    Anfoka, G; Haj Ahmad, F; Abhary, M; Hussein, A
  • Agroinoculation of Nicotiana benthamiana with cloned honeysuckle yellow vein virus isolated from Lonicera japonica
    Lee, G; Kim, S; Jung, J; Auh, C-K; Choi, E; Chang, M; Lee, S
  • Cell-to-cell and long-distance transport of viruses in plants
    Carrington, JC; Kasschau, KD; Mahajan, SK; Schaad, MC
  • Tomato dwarf leaf curl virus, a new bipartite geminivirus associated with tomatoes and peppers in Jamaica and mixed infection with tomato yellow leaf curl virus
    Roye, M; Wernecke, M; McLaughlin, W; Nakhla, M; Maxwell, D

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