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- Publisher
- Springer Journals
- Copyright
- Copyright © 2005 by Springer-Verlag/Wien
- Subject
- Biomedicine; Medical Microbiology; Virology; Infectious Diseases
- ISSN
- 0304-8608
- eISSN
- 1432-8798
- DOI
- 10.1007/s00705-004-0461-1
- pmid
- 15645376
- Publisher site
- See Article on Publisher Site
Abstract
In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.
Journal
Archives of Virology – Springer Journals
Published: May 1, 2005