Access the full text.
Sign up today, get DeepDyve free for 14 days.
S. Tang, Pyshar Yi, R. Soffe, Sofia Nahavandi, R. Shukla, K. Khoshmanesh (2015)
Using dielectrophoresis to study the dynamic response of single budding yeast cells to LyticaseAnalytical and Bioanalytical Chemistry, 407
Wen-jun Lan, W. Lan, Haiyan Wang, Lei Yan, Zhe-Li Wang (2013)
An on-bacterium flow cytometric immunoassay for protein quantification.Journal of pharmaceutical and biomedical analysis, 83
J. Jose, J. Chung, Byoung-jin Jeon, R. Maas, C. Nam, J. Pyun (2009)
Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor.Biosensors & bioelectronics, 24 5
Min Park, J. Bong, G. Yoo, J. Jose, Min‐Jung Kang, J. Pyun (2013)
Flow cytometric immunoassay using E. coli with autodisplayed Z-domains.Enzyme and microbial technology, 53 3
(2008)
Bioconjugate Techniques (2nd Edition)
J. Legras, D. Merdinoglu, J. Cornuet, F. Karst (2007)
Bread, beer and wine: Saccharomyces cerevisiae diversity reflects human historyMolecular Ecology, 16
G. Pihan (2006)
Detection of Gene Fusions in Acute Leukemia Using Bead MicroarraysCurrent Protocols in Cytometry, 35
P. Krutzik, G. Nolan (2006)
Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profilingNature Methods, 3
AC Dedent, LA Marraffini, O Schneewind (2006)
Gram-positive pathogens
A. Pandey, M. Rizvi, B. Shah, S. Bani (2016)
Anti-arthritogenic effect of Saponin-1 by alteration of Th1/Th2 cytokine paradigm in arthritic mice.Cytokine, 79
E. Harlow, David Lane (1999)
Using Antibodies: A Laboratory Manual
Ju-kyung Lee, Gu-Yoo, Min Park, J. Jose, M. Kang, J. Pyun (2012)
Electrochemical ELISA based on Escherichia coli with autodisplayed Z-domainsSensors and Actuators B-chemical, 175
M. Sjöroos, J. Ilonen, T. Lövgren (2001)
Solid-phase PCR with hybridization and time-resolved fluorometry for detection of HLA-B27.Clinical chemistry, 47 3
Y. Bae, B. Oh, Woochang Lee, W. Lee, Jeong-woo Choi (2005)
Study on orientation of immunoglobulin G on protein G layer.Biosensors & bioelectronics, 21 1
Andrea DeDent, L. Marraffini, O. Schneewind (2006)
Staphylococcal Sortases and Surface Proteins
Wen-jun Lan, Gao-Ke Hao, J. Wang, Ruihua Zhang, Wei Lan, Ruihai Wang, Rui Sun, Teng-Fei Wang (2010)
Duplexed on-microbead binding assay for competitive inhibitor of epidermal growth factor receptor by quantitative flow cytometry.Basic & clinical pharmacology & toxicology, 107 1
O. Siiman, A. Burshteyn, Maria Insausti (2001)
Covalently Bound Antibody on Polystyrene Latex Beads: Formation, Stability, and Use in Analyses of White Blood Cell Populations.Journal of colloid and interface science, 234 1
J. Robinson, Z. Darżynkiewicz, R. Hoffman, J. Nolan, P. Rabinovitch, Simon Watkins (1997)
Current Protocols In Cytometry
G. Anderson, Meagan Jacoby, F. Ligler, Keeley King (1997)
Effectiveness of protein A for antibody immobilization for a fiber optic biosensor.Biosensors & bioelectronics, 12 4
E. Diamandis, B. Hoffman, C. Sturgeon (2008)
National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines for the Use of Tumor Markers.Clinical chemistry, 54 11
A. Carmon, T. Vision, S. Mitchell, T. Thannhauser, U. Müller, S. Kresovich (2002)
Solid-phase PCR in microwells: effects of linker length and composition on tethering, hybridization, and extension.BioTechniques, 32 2
Our laboratory had developed a cell-based bio-bead for protein quantification. However, the selection of antibody in the above immunoassay is limited. This study describes a surface-decorated Saccharomyces cerevisiae for flow cytometric array immunoassay. S. cerevisiae was labeled with fluorescein isothiocyanate (FITC) and oxidized by sodium periodate, in which the saccharide group on the cytoderm outer layer was converted to an aldehyde group. In succession, adipic dihydrazide was bio-conjugated to the aldehyde group and glutaraldehyde bound to the hydrazide group. Phycoerythrin (PE)-labeled goat anti-mouse polyclonal antibody was used to assess the conjugation of mouse anti-human monoclonal antibody to surface-decorated S. cerevisiae. Cytokeratin 19 fragment (Cyfra21-1) and neuron-specific enolase (NSE) antigens were also employed to evaluate the flow cytometric array immunoassay based on surface-decorated S. cerevisiae. Flow cytometry demonstrated that FITC-barcoded S. cerevisiae as two legible populations. PE-labeled polyclonal antibody validated the coating of surface-decorated S. cerevisiae with the monoclonal antibody. The flow cytometric array immunoassays for Cyfra21-1 and NSE documented that the limit of detection (LOD) was at least 0.4 ng/mL. Precision and accuracy assessments appeared that the relative standard deviation (R.S.D.) was <15%, and the relative error (R.E.) ranged from 0.9 to 1.1. The correlation coefficient between this immunoassay and electrochemiluminescence immunoassay was 0.9622 for serum Cyfra21-1 and 0.9918 for serum NSE. In conclusion, the surface-decorated S. cerevisiae may be of use in flow cytometric array immunoassay.
Analytical and Bioanalytical Chemistry – Springer Journals
Published: Jul 4, 2017
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.