Suppressor of RNA silencing encoded by the monopartite tomato leaf curl Java begomovirus

Suppressor of RNA silencing encoded by the monopartite tomato leaf curl Java begomovirus We previously isolated the monopartite begomovirus tomato leaf curl Java virus (ToLCJAV) and satellite DNAβ02 from the same naturally infected tomato source in Indonesia. ToLCJAV induced mild leaf curl symptoms in Nicotiana benthamiana plants; DNAβ02 encoded the β C1 gene and produced severe leaf curl symptoms when co-inoculated with ToLCJAV in N. benthamiana . However, DNAβ02mβC1, which contains a frame shift mutation, did not induce severe symptoms in N. benthamiana when co-inoculated with ToLCJAV. Expression of the β C1 gene in N. benthamiana using a potato virus X (PVX) vector induced virus-like symptoms in the absence of ToLCJAV infection. When β C1 and green fluorescent protein ( GFP ) genes were co-expressed in the GFP-expressing N. benthamiana line 16c from a PVX vector, βC1 was able to suppress posttranscriptional gene silencing (PTGS) induced by GFP and eliminated the short interfering RNA (siRNA) associated with GFP expression, with a correlated increase in GFP mRNA accumulation. When C2 or C4 genes of ToLCJAV and the GFP gene were co-expressed in the GFP-expressing N. benthamiana line 16c, C2 showed a weak suppressor activity and C4 was unable to suppress PTGS induced by GFP, and siRNA associated with GFP was detected. The results of the sub-cellular localization of ToLCJAV-βC1 in the epidermal cells of N. benthamiana and onion tissues showed that this protein is accumulated towards the periphery of the cell. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Suppressor of RNA silencing encoded by the monopartite tomato leaf curl Java begomovirus

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Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-007-0957-6
Publisher site
See Article on Publisher Site

Abstract

We previously isolated the monopartite begomovirus tomato leaf curl Java virus (ToLCJAV) and satellite DNAβ02 from the same naturally infected tomato source in Indonesia. ToLCJAV induced mild leaf curl symptoms in Nicotiana benthamiana plants; DNAβ02 encoded the β C1 gene and produced severe leaf curl symptoms when co-inoculated with ToLCJAV in N. benthamiana . However, DNAβ02mβC1, which contains a frame shift mutation, did not induce severe symptoms in N. benthamiana when co-inoculated with ToLCJAV. Expression of the β C1 gene in N. benthamiana using a potato virus X (PVX) vector induced virus-like symptoms in the absence of ToLCJAV infection. When β C1 and green fluorescent protein ( GFP ) genes were co-expressed in the GFP-expressing N. benthamiana line 16c from a PVX vector, βC1 was able to suppress posttranscriptional gene silencing (PTGS) induced by GFP and eliminated the short interfering RNA (siRNA) associated with GFP expression, with a correlated increase in GFP mRNA accumulation. When C2 or C4 genes of ToLCJAV and the GFP gene were co-expressed in the GFP-expressing N. benthamiana line 16c, C2 showed a weak suppressor activity and C4 was unable to suppress PTGS induced by GFP, and siRNA associated with GFP was detected. The results of the sub-cellular localization of ToLCJAV-βC1 in the epidermal cells of N. benthamiana and onion tissues showed that this protein is accumulated towards the periphery of the cell.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 2007

References

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