Subcellular Localization of Oxaloacetate Decarboxylase and Its Isolation from Maize Leaves

Subcellular Localization of Oxaloacetate Decarboxylase and Its Isolation from Maize Leaves The activity of oxaloacetate decarboxylase was revealed in leaves of a C4 plant, maize (Zea mays L.). This activity was unrelated to decarboxylase activities of other enzymes, e.g., NAD-malate dehydrogenase (EC or NADP-malate dehydrogenase (EC, and was located in chloroplasts (83.1%). Using a four-step purification procedure, an electrophoretically pure enzyme preparation of oxaloacetate decarboxylase was obtained from maize leaves. The specific activity of the enzyme was 3.150 EU/mg protein, the factor of purification was 40.4, and the yield was 11.0%. The enzyme exhibited Michaelis–Menten kinetics with K m for oxaloacetate 30 ± 5 μM and pH optimum 7.1 ± 0.5. The metabolite-mediated regulation of oxaloacetate decarboxylase activity has been investigated. It is found that sodium chloride (1.0 mM) activates the enzyme, whereas ATP inhibits the enzyme activity. Russian Journal of Plant Physiology Springer Journals

Subcellular Localization of Oxaloacetate Decarboxylase and Its Isolation from Maize Leaves

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Kluwer Academic Publishers-Plenum Publishers
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Life Sciences; Plant Sciences
Publisher site
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