Marine microalga Isochrysis sp. was isolated from Hai Phong area of Vietnam coastal zone. Their pure cultures were raised in the laboratory for culture optimization, molecular identification and biochemical analysis. Based on the morphological studies the newly isolated species was identified as Isochrysis galbana Parke and was confirmed by 18S rRNA gene analysis. The nucleotide sequence of partial 18S rRNA gene was submitted to the GENEBANK with accession number FJ536744. Optimum conditions for growth of the species was f/2 medium at temperature 25 ± 1°C, salinity 30 ± 1 psu, pH 7.0 and light intensity of 100 μmol m−2 s−1. Under these optimum conditions, the content of protein, carbohydrate and total lipid were 27.981 ± 0.892%, 25.263 ± 0.789% and 9.780 ± 0.120% of dry cell weight, respectively (equivalent 38.768 ± 0.892%, 35.000 ± 0.789% and 13.550 ± 0.120% of ash free dry weight). I. galbana Parke, strain HP has the highest content of docosahexanenoic acid (DHA), up to 14.7% of total fatty acid and maximal polyunsaturated fatty acids (PUFAs) values at the early stationary phase in f/2 medium. The n-3 and n-6 PUFA ratio (n-3/n-6) of strain HP was from 4.50 to 5.69 at different phases of growth curve. These results suggest that biomass of this strain can be used as live feed in aquaculture.
Russian Journal of Marine Biology – Springer Journals
Published: Jul 7, 2015
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